GENOTYPIC AND ANTIGENIC IDENTIFICATION OF 2 NEW STRAINS OF SPOTTED-FEVER GROUP RICKETTSIAE ISOLATED FROM CHINA
GENOTYPIC AND ANTIGENIC IDENTIFICATION OF 2 NEW STRAINS OF SPOTTED-FEVER GROUP RICKETTSIAE ISOLATED FROM CHINA
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DOI：
10.1128/jcm.31.1.83-88.1993
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发表时间：
1993-01-01
影响因子：
9.4
通讯作者：
RAOULT, D
中科院分区：
文献类型：
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作者：
YU, XJ;JIN, Y;RAOULT, D
采用免疫荧光法、DNA聚合酶链反应-限制性内切酶片段长度多态性分析、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和Western免疫印迹法，将4株从中国蜱中分离的斑点热组立克次体与所有已知斑点热组立克次体种、株进行比较。中国分离株分三种类型，包括一种以前未被描述的新型血清型。从内蒙古纳塔利革螨蜱卵中分离得到一株与西伯利亚立克次体抗原和基因型相同的分离株。从北京采集的两株中华革螨完全相同，不同于斑点热组立克次体的其他成员，但与西伯利亚恙螨明显密切相关。HA-91是一株从亚洲透明瘤中分离出来的菌株。在斑点热组立克次体中，Kozlovi olenew在抗原性和基因典型上是独特的，我们认为这里提出的数据应该促使人们在当前立克次体分类的基础上考虑将其作为一个新物种。
Four isolates of spotted fever group rickettsiae isolated from ticks in China were compared with all known species and strains of spotted fever group rickettsiae by immunofluorescence assay, DNA polymerase chain reaction followed by restriction endonuclease fragment length polymorphism analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western immunoblot. The Chinese isolates belonged to three types, including a novel serotype which has not been described before. One isolate obtained from tick ova of Dermacentor nuttallii in Inner Mongolia was antigenically and genotypically identical to Rickettsia sibirica. Two isolates obtained from Dermacentor sinicus collected from Beijing were identical, different from other members of spotted fever group rickettsiae but apparently closely related to R. sibirica. HA-91, a strain isolated from Hyalomma asiaticum bv. kozlovi olenew, was antigenically and genotypically unique among spotted fever group rickettsiae, and we feel that data presented here should prompt consideration of it as a new species on the basis of current rickettsial taxonomy.