Effect of riboflavin on the photolysis of folic acid in aqueous solution.
Effect of riboflavin on the photolysis of folic acid in aqueous solution.
复制标题
核黄素对水溶液中叶酸光解的影响。
DOI：
10.1016/s0731-7085(00)00383-6
复制
发表时间：
2000
影响因子：
3.4
通讯作者：
Iqbal Ahmad
中科院分区：
文献类型：
--
作者：
M. Akhtar;M.Ataullah Khan;Iqbal Ahmad
本文研究了核黄素存在下叶酸在水溶液中的可见光光解。在pH 4.0-9.0范围内测定了叶酸和核黄素双分子相互作用的二级速率常数。速率-pH曲线显示速率逐渐增加，直至pH 6.2（pHmax.）随后降低至pH 9.0，这取决于参与相互作用的离子种类的敏感性。在所研究的pH范围内，光解速率从0.50× 103 M − 1 min −1（pH 9.0）和0.63× 103 M − 1 min −1（pH 4.0）到3.0× 103 M − 1 min −1（pH 6.2）不等。本文采用高效液相色谱法测定了核黄素存在下叶酸及其光产物蝶呤-6-羧酸和对氨基苯甲酰-L-谷氨酸的含量。
A study of the photolysis of folic acid in aqueous solution by visible radiation in the presence of riboflavin has been made. The second-order rate constants for the bimolecular interaction of folic acid and riboflavin have been determined in the pH range 4.0–9.0. The rate–pH profile shows a gradual increase in the rate up to pH 6.2 (pHmax.) followed by a decrease up to pH 9.0, depending upon the susceptibility of ionic species involved in the interaction. The rate of photolysis varies from 0.50×103M−1min−1(pH 9.0) and 0.63×103M−1min−1(pH 4.0) to 3.0×103M−1min−1(pH 6.2) in the pH range studied. A HPLC method has been used for the assay of folic acid and its photoproducts, pterine-6-carboxylic acid and p-aminobenzoyl-l-glutamic acid in the presence of riboflavin.