MoLEU1, MoLEU2, and MoLEU4 regulated by MoLEU3 are involved in leucine biosynthesis, fungal development, and pathogenicity in Magnaporthe oryzae
MoLEU1, MoLEU2, and MoLEU4 regulated by MoLEU3 are involved in leucine biosynthesis, fungal development, and pathogenicity in Magnaporthe oryzae
复制标题
受 MoLEU3 调控的 MoLEU1、MoLEU2 和 MoLEU4 参与稻瘟病菌的亮氨酸生物合成、真菌发育和致病性
DOI：
10.1111/1758-2229.12800
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发表时间：
2019
影响因子：
3.3
通讯作者：
Liang Shuang
中科院分区：
文献类型：
--
作者：
Wei Yun-Yun;Yu Qin;Dong Bo;Zhang Yong;Liu Xiao-Hong;Lin Fu-Cheng;Liang Shuang
氨基酸是细胞新陈代谢的重要组成部分。亮氨酸是一种调节因子，对蛋白质合成/周转产生显著影响，调节多种细胞信号传导途径，并参与氧化过程和免疫反应。在这里，我们确定并表征了亮氨酸相关的Zn 2Cys 6型转录因子MoLeu 3的功能。MoLEU 3的破坏导致大麦和水稻的致病性显着降低。定量RT-PCR结果显示，亮氨酸生物合成相关基因MoLEU 1、MoLEU 2和MoLEU 4在Δ Moleu 3突变体中表达下调。我们采用高通量基因敲除方法分别获得MoLEU 1、MoLEU 2和MoLEU 4的无效突变体。Δ Moleu 1、Δ Moleu 2和Δ Moleu 4突变体均为亮氨酸营养缺陷型，表现出相似的表型特征，包括产孢减少、糖原和脂滴的动员和降解延迟、附着胞介导的渗透有限以及侵入性菌丝在宿主细胞内的生长受限。MoLEU 3调控的MoLEU 1、MoLEU 2和MoLEU 4通过调节稻瘟病菌亮氨酸的生物合成在真菌发育和感染过程中发挥重要作用。
Amino acids are vital components in cell metabolism. Leucine is a regulatory factor that generates significant impact on protein synthesis/turnover, modulates diverse cellular signalling pathways and participates in oxidative processes and immune responses. Here, we identified and characterized the functions of a leucine‐associated Zn2Cys6‐type transcription factor, MoLeu3. Disruption ofMoLEU3resulted in significantly reduced pathogenicity in barley and rice. Quantitative RT‐PCR showed that the expression levels of the putative leucine biosynthesis‐related genes,MoLEU1,MoLEU2andMoLEU4were downregulated in the ΔMoleu3mutant. We used high‐throughput gene knockout method to generate the null mutants ofMoLEU1,MoLEU2andMoLEU4respectively. The ΔMoleu1, ΔMoleu2and ΔMoleu4mutants are leucine auxotroph and showed similar phenotypic characterizations, including reduced conidiation, delayed mobilization and degradation of glycogen and lipid droplets, limited appressorium‐mediated penetration, and restricted invasive hyphae growth within host cells. Collectively,MoLEU1,MoLEU2, andMoLEU4regulated byMoLEU3play crucial roles in fungal development and infectious processes through modulation of leucine biosynthesis inMagnaporthe oryzae.