Sprayable hydrogel dressing accelerates wound healing with combined reactive oxygen species-scavenging and antibacterial abilities
Sprayable hydrogel dressing accelerates wound healing with combined reactive oxygen species-scavenging and antibacterial abilities
复制标题
可喷雾水凝胶敷料具有活性氧清除和抗菌能力，可加速伤口愈合
DOI：
10.1016/j.actbio.2021.02.002
复制
发表时间：
2021-03-20
影响因子：
9.7
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Cheng, Hao;Shi, Zhe;Wang, Jian
考虑到伤口感染、延迟愈合和瘢痕形成的影响，伤口管理对全球医疗保健系统造成相当大的经济负担。为此，已经开发了基于水凝胶的多功能敷料以刺激皮肤愈合。在此，我们描述了一种可喷涂的水凝胶基伤口敷料的设计、制造和表征，该敷料装载有氧化铈纳米颗粒（CeON）和抗菌肽（AMP），用于结合活性氧（ROS）清除和抗菌特性。我们采用贻贝启发的策略，将明胶与多巴胺基序化学结合，并制备了对湿皮肤表面具有改善的结合亲和力的水凝胶敷料。此外，AMP从水凝胶中的释放证明了对四种代表性细菌菌株的快速释放消融和接触消融，证实了所需的抗微生物活性。此外，负载CeONs的水凝胶敷料表现出良好的ROS清除能力。通过与HaCaT细胞一起培养，进一步证明了多功能水凝胶敷料的生物相容性。总的来说，所开发的水凝胶伤口敷料的益处，包括可喷涂性、可渗透性、抗微生物活性以及ROS清除和皮肤重塑能力，突出了其在伤口管理中的有希望的转化潜力。考虑到伤口感染、延迟愈合和瘢痕形成的影响，伤口管理对全球医疗保健系统造成了相当大的经济负担。为此，已经开发了基于水凝胶的多功能敷料以刺激皮肤愈合。在此，我们描述了一种可喷涂的水凝胶基伤口敷料的设计、制造和表征，该敷料装载有氧化铈纳米颗粒（CeON）和抗菌肽（AMP），用于结合活性氧（ROS）清除和抗菌特性。我们采用贻贝启发的策略，将明胶与多巴胺基序化学结合，并制备了对湿皮肤表面具有改善的结合亲和力的水凝胶敷料。此外，AMP从水凝胶中的释放证明了对四种代表性细菌菌株的快速释放消融和接触消融，证实了所需的抗微生物活性。此外，负载CeONs的水凝胶敷料表现出良好的ROS清除能力。通过与HaCaT细胞一起培养，进一步证明了多功能水凝胶敷料的生物相容性。总的来说，开发的水凝胶伤口敷料的益处，包括可喷涂性、抗微生物活性、以及ROS清除和皮肤重塑能力，突出了其在伤口管理中的有希望的转化潜力。然而，从临床角度来看，目前的伤口敷料很少能满足所有预期的多功能要求，即预防感染、促进伤口快速闭合和最大限度地减少瘢痕形成，同时提供应用便利性。在本研究中，我们采用贻贝启发的策略用DOPA功能化GelMA水凝胶以制备GelMA-DOPA水凝胶，其对伤口表面表现出增强的结合亲和力，AMP HHC-36和CeON进一步包封到GelMA-DOPA水凝胶中以赋予水凝胶伤口敷料抗菌和ROS清除能力。GelMA-DOPA-AMP-CeONs敷料具有可喷涂性、抗菌性、抗微生物活性以及ROS清除和皮肤重塑能力等优点，可解决与慢性伤口治疗和管理相关的治疗和经济负担。(c)2021 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。各种基于水凝胶的伤口敷料材料已被开发出来，以刺激伤口愈合。然而，从临床角度来看，目前的伤口敷料很少能满足所有预期的多功能要求，即预防感染、促进伤口快速闭合和最大限度地减少瘢痕形成，同时提供应用便利性。在本研究中，我们采用贻贝启发的策略用DOPA功能化GelMA水凝胶以制备GelMA-DOPA水凝胶，其对伤口表面表现出增强的结合亲和力，AMP HHC-36和CeON进一步包封到GelMA-DOPA水凝胶中以赋予水凝胶伤口敷料抗菌和ROS清除能力。GelMA-DOPA-AMP-CeONs敷料具有可喷涂性、抗菌性、抗微生物活性以及ROS清除和皮肤重塑能力等优点，可解决与慢性伤口治疗和管理相关的治疗和经济负担。
Wound management poses a considerable economic burden on the global healthcare system, considering the impacts of wound infection, delayed healing and scar formation. To this end, multifunctional dressings based on hydrogels have been developed to stimulate skin healing. Herein, we describe the design, fabrication, and characterization of a sprayable hydrogel-based wound dressing loaded with cerium oxide nanoparticles (CeONs) and an antimicrobial peptide (AMP), for combined reactive oxygen species (ROS)-scavenging and antibacterial properties. We adopted a mussel-inspired strategy to chemically conjugate gelatin with dopamine motifs and prepared a hydrogel dressing with improved binding affinity to wet skin surfaces. Additionally, the release of AMP from the hydrogel demonstrated rapid release ablation and contact ablation against four representative bacterial strains, confirming the desired antimicrobial activities. Moreover, the CeONs-loaded hydrogel dressing exhibited favorable ROS-scavenging abilities. The biocompatibility of the multifunctional hydrogel dressing was further proven in vitro by culturing with HaCaT cells. Overall, the benefits of the developed hydrogel wound dressing, including sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, highlight its promissing translational potentials in wound management.Wound management poses a considerable economic burden on the global healthcare system, considering the impacts of wound infection, delayed healing and scar formation. To this end, multifunctional dressings based on hydrogels have been developed to stimulate skin healing. Herein, we describe the design, fabrication, and characterization of a sprayable hydrogel-based wound dressing loaded with cerium oxide nanoparticles (CeONs) and an antimicrobial peptide (AMP), for combined reactive oxygen species (ROS)-scavenging and antibacterial properties. We adopted a mussel-inspired strategy to chemically conjugate gelatin with dopamine motifs and prepared a hydrogel dressing with improved binding affinity to wet skin surfaces. Additionally, the release of AMP from the hydrogel demonstrated rapid release ablation and contact ablation against four representative bacterial strains, confirming the desired antimicrobial activities. Moreover, the CeONs-loaded hydrogel dressing exhibited favorable ROS-scavenging abilities. The biocompatibility of the multifunctional hydrogel dressing was further proven in vitro by culturing with HaCaT cells. Overall, the benefits of the developed hydrogel wound dressing, including sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, highlight its promissing translational potentials in wound management.Statement of SignificanceVarious hydrogel-based wound-dressing materials have been developed to stimulate wound healing. However, from the clinical perspective, few of the current wound dressings meet all the intended multifunctional requirements of preventing infection, promoting rapid wound closure, and minimizing scar formation, while simultaneously offering the convenience of application. In the current study, we adopted a mussel-inspired strategy to functionalize the GelMA hydrogels with DOPA to fabricate GelMA-DOPA hydrogel which exhibited an enhanced binding affinity for wound surfaces, AMP HHC-36 and CeONs are further encapsulated into the GelMA-DOPA hydrogel to confer the hydrogel wound dressing with antimicrobial and ROS-scavenging abilities. The GelMA-DOPA-AMP-CeONs dressing offered the benefits of sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, which might address the therapeutic and economic burdens associated with chronic wound treatment and management. (c) 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.Various hydrogel-based wound-dressing materials have been developed to stimulate wound healing. However, from the clinical perspective, few of the current wound dressings meet all the intended multifunctional requirements of preventing infection, promoting rapid wound closure, and minimizing scar formation, while simultaneously offering the convenience of application. In the current study, we adopted a mussel-inspired strategy to functionalize the GelMA hydrogels with DOPA to fabricate GelMA-DOPA hydrogel which exhibited an enhanced binding affinity for wound surfaces, AMP HHC-36 and CeONs are further encapsulated into the GelMA-DOPA hydrogel to confer the hydrogel wound dressing with antimicrobial and ROS-scavenging abilities. The GelMA-DOPA-AMP-CeONs dressing offered the benefits of sprayability, adhesiveness, antimicrobial activity, as well as ROS-scavenging and skin-remodeling ability, which might address the therapeutic and economic burdens associated with chronic wound treatment and management.