Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic Analysis
Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic Analysis
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DOI：
10.1021/acs.jafc.7b06146
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发表时间：
2018-04-04
影响因子：
6.1
通讯作者：
Luo, Zisheng
中科院分区：
文献类型：
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作者：
Feng, Simin;Gan, Ling;Luo, Zisheng
为了研究豆甾醇和β-谷甾醇对高脂西餐(HFWD)诱导的非酒精性脂肪性肝病(NAFLD)的影响，对治疗33周后采集的肝脏样本进行了脂组学分析。主成分分析表明，这些植物甾醇对HFWD诱导的NAFLD具有有效的保护作用。潜在结构的正交投影判别分析和S图解表明，三酰甘油、磷脂酰胆碱、胆固醇酯、二酰甘油和游离脂肪酸是造成这些判别的主要脂肪物种。NAFLD的缓解主要与降低肝脏胆固醇、多不饱和脂肪酸的甘油三酯和改变肝脏游离FFA有关。总之，在这项长期(33周)的研究中，植物甾醇的剂量与FDA建议的人类降低血浆胆固醇水平的剂量相当，被证明可以预防NAFLD。
To study the effects of stigmasterol and beta-sitosterol on high-fat Western diet (HFWD)-induced nonalcoholic fatty liver disease (NAFLD), lipidomic analyses were conducted in liver samples collected after 33 weeks of the treatment. Principal component analysis showed these phytosterols were effective in protecting against HFWD-induced NAFLD. Orthogonal projections to latent structures-discriminate analysis (OPLS-DA) and S-plots showed that triacylglycerols (TGs), phosphatidylcholines, cholesteryl esters, diacylglycerols, and free fatty acids (FFAs) were the major lipid species contributing to these discriminations. The alleviation of NAFLD is mainly associated with decreases in hepatic cholesterol, TGs with polyunsaturated fatty acids, and alterations of free hepatic FFA. In condusion, phytosterols, at a dose comparable to that suggested for humans by the FDA for the reduction of plasma cholesterol levels, are shown to protect against NAFLD in this long-term (33-week) study.