Coronary microembolization sheep model by adjusting the number of microspheres based on coronary blood flow
Coronary microembolization sheep model by adjusting the number of microspheres based on coronary blood flow
复制标题
根据冠状动脉血流量调整微球数量的冠状动脉微栓塞羊模型
DOI：
10.1111/aor.14385
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发表时间：
2023
影响因子：
2.4
通讯作者：
Minoru Ono
中科院分区：
文献类型：
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作者：
Shun Tanaka;Tomohiro Nishinaka;Akihide Umeki;Masahiko Fujii;Shusuke Imaoka;Futoshi Kobayashi;Ayako Inatomi;Nobumasa Katagiri;Tomonori Tsukiya;Toshihide Mizuno;Minoru Ono
背景使用大型动物冠状动脉微栓塞的心力衰竭（HF）模型对于医学研究是必不可少的。然而，心肌对微栓塞反应的异质性是一个限制。我们假设根据冠状动脉血流量调整注射微球的数量可以稳定心力衰竭的严重程度。本研究旨在根据动物模型的左冠状动脉血流量来评价微球注射的效果。方法通过向两组羊的左冠状动脉左降支注射不同数量的微球（聚苯乙烯，直径：90μm）（400和600倍冠状动脉血流[ml/min]）诱导微栓塞。微栓塞后0.5、1.5、3.5、6.5小时检查血流动力学参数、左心室压力-容量环和超声心动图结果。结果舒张末压和标准化心率随时间增加，600 × 冠状动脉血流组显着高于400 × 冠状动脉血流组（p= 0.04 且 p< 0.01， 分别）。左心室压力升高的最大速率和归一化每搏输出量随着时间的推移而降低，并且600 × 冠状动脉血流量组显着低于400 × 冠状血流量组（分别为p< 0.01和p< 0.01）。每冠状动脉血流量的微球数量与每搏输出量的减少和6.5 h内左心室压力上升的最大速率显着相关（分别为r= 0.74，p= 0.01和r= 0.71，p= 0.02）。结论根据冠状动脉血流量调整注射微球的数量，可以创建不同严重程度的心力衰竭模型。
BackgroundA heart failure (HF) model using coronary microembolization in large animals is indispensable for medical research. However, the heterogeneity of myocardial response to microembolization is a limitation. We hypothesized that adjusting the number of injected microspheres according to coronary blood flow could stabilize the severity of HF. This study aimed to evaluate the effect of microsphere injection based on the left coronary artery blood flow in an animal model.MethodsMicroembolization was induced by injecting different numbers of microspheres (polystyrene, diameter: 90 μm) into the left descending coronary artery of the two groups of sheep (400 and 600 times coronary blood flow [ml/min]). Hemodynamic parameters, the pressure–volume loop of the left ventricle, and echocardiography findings were examined at 0.5, 1.5, 3.5, and 6.5 h after microembolization.ResultsEnd‐diastolic pressure and normalized heart rate increased over time, and were significantly higher in 600 × coronary blood flow group than those in 400 × coronary blood flow group (p= 0.04 andp< 0.01, respectively). The maximum rate of left‐ventricular pressure rise and normalized stroke volume decreased over time, and were significantly lower in 600 × coronary blood flow group than those in 400 × coronary blood flow group (p< 0.01 andp< 0.01, respectively). The number of microspheres per coronary blood flow was significantly correlated with the decrease in stroke volume and the maximum rate of left ventricular pressure rise in 6.5 h (r= 0.74,p= 0.01 andr= 0.71,p= 0.02, respectively).ConclusionsAdjusting the number of injected microspheres based on the coronary blood flow enabled the creation of HF models with different degrees of severity.