Rapid Characterization of Microalgae and Microalgae Mixtures Using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS).
Rapid Characterization of Microalgae and Microalgae Mixtures Using Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS).
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DOI：
10.1371/journal.pone.0135337
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Dempster T
中科院分区：
文献类型：
--
作者：
Barbano D;Diaz R;Zhang L;Sandrin T;Gerken H;Dempster T
目前表征微藻的分子方法既耗时又昂贵。基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF MS)可能是一种快速、经济的替代方法。这项研究的目的是确定MALDI-TOF MS是否可以用于：1)在物种和菌株水平上区分微藻；2)表征简单的微藻混合物。采用一种常用的蛋白质提取样品前处理方法，对31种微藻进行了快速的质谱分析。每个产生的光谱都包含6到56个峰，在m/z 2000到20,000范围内。这种方法的分类分辨率似乎高于18S rDNA序列分析。例如，两个品系的尖吻栅藻只有两个18S rDNA核苷酸的差异，但产生了不同的MALDI-TOF质谱图。两种和三种微藻的混合物产生了相对复杂的光谱，其中包含与每个混合物的成员相关的峰。然而，有趣的是，在m/z 11,048和11,230处观察到了混合物特有的峰。我们的结果表明，MALDI-TOF MS提供了单个微藻和简单微藻混合物的快速表征。
Current molecular methods to characterize microalgae are time-intensive and expensive. Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) may represent a rapid and economical alternative approach. The objectives of this study were to determine whether MALDI-TOF MS can be used to: 1) differentiate microalgae at the species and strain levels and 2) characterize simple microalgal mixtures. A common protein extraction sample preparation method was used to facilitate rapid mass spectrometry-based analysis of 31 microalgae. Each yielded spectra containing between 6 and 56 peaks in the m/z 2,000 to 20,000 range. The taxonomic resolution of this approach appeared higher than that of 18S rDNA sequence analysis. For example, two strains of Scenedesmus acutus differed only by two 18S rDNA nucleotides, but yielded distinct MALDI-TOF mass spectra. Mixtures of two and three microalgae yielded relatively complex spectra that contained peaks associated with members of each mixture. Interestingly, though, mixture-specific peaks were observed at m/z 11,048 and 11,230. Our results suggest that MALDI-TOF MS affords rapid characterization of individual microalgae and simple microalgal mixtures.