Effects of lutein and cholesterol on alkyl chain bending in lipid bilayers: a pulse electron spin resonance spin labeling study.
Effects of lutein and cholesterol on alkyl chain bending in lipid bilayers: a pulse electron spin resonance spin labeling study.
复制标题
叶黄素和胆固醇对脂质双层烷基链弯曲的影响：脉冲电子自旋共振自旋标记研究。
DOI：
10.1016/s0006-3495(96)79284-6
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发表时间：
1996
影响因子：
3.4
通讯作者：
Subczynski,WK
中科院分区：
文献类型：
--
作者：
Yin,JJ;Subczynski,WK
采用短脉冲饱和恢复电子自旋共振技术研究了极性类胡萝卜素-叶黄素和胆固醇对液相磷脂酰胆碱（PC）膜中14N:15N硬脂酸自旋标记对相互作用的影响。在27℃下测量了由[14N]-16-， [14N]-10-, [14N]-7-，或[14N]-5-羟硬脂酸酯和[15N]-16-羟硬脂酸酯组成的双分子对撞在二肉豆醇-PC （DMPC）或蛋黄PC （EYPC）膜中的双分子碰撞。在两种脂质系统中叶黄素或胆固醇的缺失和存在情况下，碰撞率为16:5< 16:7< 16:10< 16:16。在所研究的所有自旋标签对中，DMPC中的相互作用频率大于EYPC。极性类胡萝卜素-叶黄素降低了所有自旋标签对的碰撞频率，而胆固醇降低了16:5和16:7对的碰撞频率，增加了16:10和16:16对膜中心的碰撞频率。不饱和烷基链的存在大大降低了叶黄素的作用，但放大了膜中心胆固醇的作用。所观察到的这些改性剂对烷基链弯曲影响的差异是由于胆固醇和极性类胡萝卜素的结构不同以及它们在脂质双分子层膜内的不同定位。这些研究进一步证实了烷基链末端向双层表面垂直波动的存在。
A short pulse saturation recovery electron spin resonance technique has been used to study the effects of polar carotenoid-lutein and cholesterol on interactions of 14N:15N stearic acid spin-label pairs in fluid-phase phosphatidylcholine (PC) membranes. Bimolecular collisions for pairs consisting of various combinations of [14N]-16-, [14N]-10-, [14N]-7-, or [14N]-5-doxylstearate and [15N]-16-doxylstearate in dimyristoyl-PC (DMPC) or egg yolk PC (EYPC) membranes were measured at 27 degrees C. In the absence and presence of lutein or cholesterol for both lipid systems, the collision rates were ordered as 16:5< 16:7< 16:10< 16:16. For all spin-label pairs studied, interaction frequencies were greater in DMPC than in EYPC. Polar carotenoid-lutein reduces the collision frequency for all spin-label pairs, whereas cholesterol reduces the collision frequency for 16:5 and 16:7 pairs and increases the collision frequency in the membrane center for 16:10 and 16:16 pairs. The presence of unsaturated alkyl chains greatly reduces the effect of lutein but magnifies the effect of cholesterol in the membrane center. The observed differences in the effects of these modifiers on alkyl chain bending result from differences in the structure of cholesterol and polar carotenoid and from their different localization within the lipid bilayer membrane. These studies further confirm the occurrence of vertical fluctuations of alkyl chain ends toward the bilayer surface.