Over-expression of PPARα in obese mice adipose tissue improves insulin sensitivity
Over-expression of PPARα in obese mice adipose tissue improves insulin sensitivity
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DOI：
10.1016/j.bbrc.2017.09.067
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发表时间：
2017-11-04
影响因子：
3.1
通讯作者：
Goto, Tsuyoshi
中科院分区：
文献类型：
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作者：
Takahashi, Haruya;Sanada, Kohei;Goto, Tsuyoshi
过氧化物酶体增殖物激活受体 a (PPAR α) 在脂质代谢调节中发挥重要作用，并在肝脏中高水平表达。尽管PPARα也在脂肪组织中表达，但对其激活与葡萄糖代谢调节之间的关系知之甚少。在这项研究中，我们培育了脂肪组织特异性 PPARa 过表达 (OE) 小鼠。代谢组学和胰岛素耐受性测试表明，OE 可诱导支链氨基酸 (BCAA) 分布并改善胰岛素敏感性。此外，LC-MS 和 PCR 分析表明，OE 可以改变游离脂肪酸 (FFA) 谱并减少肥胖引起的炎症。这些发现表明，脂肪组织中的 PPAR α 激活有助于通过增强 BCAA 和 FFA 代谢来改善葡萄糖代谢紊乱。 (C) 2017 Elsevier Inc. 保留所有权利。
Peroxisome proliferator-activated receptor a (PPAR alpha) is important in the regulation of lipid metabolism and expressed at high levels in the liver. Although PPAR alpha is also expressed in adipose tissue, little is known about the relationship between its activation and the regulation of glucose metabolism. In this study, we developed adipose tissue specific PPARa over-expression (OE) mice. Metabolomics and insulin tolerance tests showed that OE induces branched-chain amino acid (BCAA) profile and improvement of insulin sensitivity. Furthermore, LC-MS and PCR analyses revealed that OE changes free fatty acid (FFA) profile and reduces obesity-induced inflammation. These findings suggested that PPAR alpha activation in adipose tissue contributes to the improvement of glucose metabolism disorders via the enhancement of BCAA and FFA metabolism. (C) 2017 Elsevier Inc. All rights reserved.