Mitochondrial inheritance is required for MEN-regulated cytokinesis in budding yeast.
Mitochondrial inheritance is required for MEN-regulated cytokinesis in budding yeast.
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DOI：
10.1016/j.cub.2009.08.041
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发表时间：
2009-11-03
期刊：
影响因子：
--
通讯作者：
Pon LA
中科院分区：
文献类型：
--
作者：
García-Rodríguez LJ;Crider DG;Gay AC;Salanueva IJ;Boldogh IR;Pon LA
线粒体遗传，即在细胞分裂过程中线粒体从母体细胞转移到子体细胞，对于子体细胞的生存能力至关重要。线粒体是一种含有Mdm 10 p、Mdm 12 p和Mmm 1 p的线粒体蛋白复合物，是芽殖酵母中线粒体运动性导致遗传所必需的。我们观察到线粒体突变体和另一个线粒体遗传受损的突变体（mmr 1 Δ gem 1 Δ）的胞质分裂缺陷。在不具有线粒体DNA或线粒体外膜中的线粒体蛋白输入或β-桶蛋白组装缺陷的酵母中未观察到该缺陷。MDM 10的缺失抑制收缩环闭合，但不抑制收缩环组装、染色体过客蛋白在后期早期定位于纺锤体、纺锤体对齐、核仁分离或后期核迁移。在mdm 10 Δ细胞中，有丝分裂出口网络（MEN）组分Cdc 14 p在后期从核仁中的释放被延迟。最后，通过缺失BUB 2而过度激活MEN恢复了mdm 10 Δ和mmr 1 Δ gem 1 Δ细胞中胞质分裂的缺陷，并降低了野生型和mdm 10 Δ细胞中母细胞和子细胞之间线粒体分离的保真度。我们的研究确定了一种新的MEN相关的调节系统，该系统抑制胞质分裂以响应芽殖酵母中线粒体遗传的缺陷。
Mitochondrial inheritance, the transfer of mitochondria from mother to daughter cell during cell division, is essential for daughter cell viability. The mitochore, a mitochondrial protein complex containing Mdm10p, Mdm12p and Mmm1p, is required for mitochondrial motility leading to inheritance in budding yeast. We observe a defect in cytokinesis in mitochore mutants and another mutant (mmr1Δ gem1Δ) with impaired mitochondrial inheritance. This defect is not observed in yeast that have no mitochondrial DNA or defects in mitochondrial protein import or assembly of β-barrel proteins in the mitochondrial outer membrane. Deletion of MDM10 inhibits contractile ring closure, but does not inhibit contractile ring assembly, localization of a chromosomal passenger protein to the spindle during early anaphase, spindle alignment, nucleolar segregation or nuclear migration during anaphase. Release of the mitotic exit network (MEN) component, Cdc14p, from the nucleolus during anaphase is delayed in mdm10Δ cells. Finally, hyperactivation of the MEN by deletion of BUB2 restores defects in cytokinesis in mdm10Δ and mmr1Δ gem1Δ cells, and reduces the fidelity of mitochondrial segregation between mother and daughter cells in wild-type and mdm10Δ cells. Our studies identify a novel MEN-linked regulatory system that inhibits cytokinesis in response to defects in mitochondrial inheritance in budding yeast.
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影响因子：
5.3
作者：
Luca, FC;Mody, M;Winey, M
通讯作者：
Winey, M
CYK2（CDC15/PSTPIP家族蛋白）的双重功能在调节肌动蛋白环动力学和SEPTIN分布中。
DOI：
10.1083/jcb.143.7.1947
发表时间：
1998-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lippincott J;Li R
通讯作者：
Li R
DOI：
10.1083/jcb.152.3.451
发表时间：
2001-02-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Song S;Lee KS
通讯作者：
Lee KS
DOI：
10.1083/jcb.141.6.1371
发表时间：
1998-06-15
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boldogh I;Vojtov N;Karmon S;Pon LA
通讯作者：
Pon LA
影响因子：
4.4
作者：
Millard, PJ;Roth, BL;Haugland, RP
通讯作者：
Haugland, RP