The effect of low temperature (5–29 °C) and adaptation on the methanogenic activity of biomass
The effect of low temperature (5–29 °C) and adaptation on the methanogenic activity of biomass
复制标题
低温（5-29℃）和适应对生物质产甲烷活性的影响
DOI：
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发表时间：
1997
影响因子：
5
通讯作者：
Jukka Rintala
中科院分区：
文献类型：
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作者：
R. Kettunen;Jukka Rintala
摘要以城市垃圾渗滤液、厌氧降解中间体丙酸酯和甲烷前体乙酸酯、H2/CO2为底物，研究了低温（5 ~ 29℃）对非适应消化污泥产甲烷活性和适应温度/渗滤液的生物质的影响。产甲烷活性的温度依赖性可以用arrhenius模型来描述。然而，底物和适应性都影响了温度依赖性。在大约20°C的渗滤液上流式厌氧污泥包层反应器中，生物质适应4个月，在11°C和22°C的条件下，产甲烷活性分别提高了7倍和5倍。在5℃条件下，乙酸酯和H2/CO2均发生甲烷化。22℃时，产甲烷活性（乙酸4.8 ~ 84 mM）是11℃时的1.6 ~ 5.2倍。在11℃时醋酸酯利用率的半速度常数（Ks）是22℃时的三分之一，而在两种温度下得到的Ki相似。以丙酸酯（1.1-5.5 mM）为底物，在11°C下的甲基厌氧活性是22°C时的一半。此外，底物的残留浓度不依赖于温度。结果表明，生物质的适应性可以在厌氧过程中实现高处理能力，即使在亲湿条件下。
Abstract The influence of low temperature (5–29 °C) on the methanogenic activity of non-adapted digested sewage sludge and on temperature/leachate-adapted biomass was assayed by using municipal landfill leachate, intermediates of anaerobic degradation (propionate) and methane precursors (acetate, H2/CO2) as substrates. The temperature dependence of methanogenic activity could be described by Arrhenius-derived models. However, both substrate and adaptation affected the temperature dependence. The adaptation of biomass in a leachate-fed upflow anaerobic sludge-blanket reactor at approximately 20 °C for 4 months resulted in a sevenfold and fivefold increase of methanogenic activity at 11 °C and 22 °C respectively. Both acetate and H2/CO2 were methanized even at 5 °C. At 22 °C, methanogenic activities (acetate 4.8–84 mM) were 1.6–5.2 times higher than those at 11 °C. The half-velocity constant (Ks) of acetate utilization at 11 °C was one-third of that at 22 °C while a similar Ki was obtained at both temperatures. With propionate (1.1–5.5 mM) as substrate, meth‐anogenic activities at 11 °C were half those at 22 °C. Furthermore, the residual concentration of the substrates was not dependent on temperature. The results suggest that the adaptation of biomass enables the achievement of a high treatment capacity in the anaerobic process even under psychrophilic conditions.