Genetic Effects of Vascular Endothelial Growth Factor A (VEGF-A) and Its Association with Disease Progression in Breast Cancer Population of Saudi Arabia.
Genetic Effects of Vascular Endothelial Growth Factor A (VEGF-A) and Its Association with Disease Progression in Breast Cancer Population of Saudi Arabia.
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DOI：
10.31557/apjcp.2020.21.1.139
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发表时间：
2020-01-01
期刊：
影响因子：
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通讯作者：
Abu Duhier, F M
中科院分区：
文献类型：
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作者：
Albalawi, Ibrahim Altedlawi;Mir, Rashid;Abu Duhier, F M
目的：已有研究表明血管内皮生长因子（VEGFA）基因变异与乳腺癌风险相关。本研究的目的是评估血管内皮生长因子（VEGF）对乳腺癌风险及其与疾病进展的关系的遗传效应。方法：对110例乳腺癌病例和110例性别匹配的健康对照进行病例对照研究。采用扩增难治性突变系统PCR方法进行血管内皮生长因子A（VEGF-A）1（-460T>C）基因分型。结果：VEGF-A（-460T>C）基因型在乳腺癌组和性别匹配的健康对照组中的分布差异有统计学意义（p=0.006）。在乳腺癌患者和性别匹配的健康对照组中，CC、CT、TT三种基因型的频率分别为4.54%、46.36%、49.20%和7.27%、64.54%、28.18%。在共显性遗传模型中，VEGF（-460 T>C）CC和TT变异体与乳腺癌易感性增加相关，OR 2.78（0.83-9.26）RR 1.68（1.01 - 2.81）P=0.04。在隐性遗传模型中，VEGF（-460 T>C）（CC+CT vs. TT）变异体与VEGF（-460 T> C）显著相关（OR=2.45（95% CI=（1.40-4.29），P=0.003）。我们的研究结果表明，VEGF（-460 T>C）TT基因型与乳腺癌疾病的易感性增加有关。结论：VEGF（-460T>C）TT基因型与乳腺癌易感性有显著相关性，VEGF（-460T>C）TT基因型与乳腺癌易感性有显著相关性。VEGF-A（-460T>C）基因多态性与肿瘤远处转移密切相关。它可能是一个有用的乳腺癌易感基因标记。进一步的研究，更大的样本量是必要的，以证实我们的发现。
AIM: Previous studies have shown that vascular endothelial growth factor (VEGFA) gene variants were associated with breast cancer risk. The goal of the current study was to evaluate the genetic effects of the vascular endothelial growth factor (VEGF) on the risk of breast cancer and its association with disease progression.METHODOLOGY: This case control study was conducted on 110 Breast cancer cases and 110 gender matched healthy controls. Vascular endothelial growth factor A (VEGF-A) 1 (-460T>C) genotyping was performed using Amplification refractory mutation system PCR method. The vascular endothelial growth factor A (VEGF-A) (-460T>C) genotypes were collated with different clinicopathological features of breast cancer patients.RESULTS: A significant difference was observed between the genotype distribution of VEGF-A (-460T>C) among breast cancer cases and gender matched healthy controls (p=0.006). The frequencies of all three genotypes CC,CT,TT reported in the breast cancer patients and sex matched healthy controls were 4.54%, 46.36% ,49.20% and 7.27%, 64.54%, 28.18% respectively. The increased susceptibility to breast cancer disease was found to be associated with VEGF (-460T>C) CC vs TT variant in codominant inheritance model OR 2.78 (0.83-9.26) RR 1.68(1.01 to 2.81) P=0.04. A significant association was reported with VEGF (-460T>C) (CC+CT vs. TT) variant in recessive inheritance model, (OR=2.45 (95% CI= (1.40-4.29), P=0.003. Our findings indicated that VEGF (-460T>C) TT genotype is associated with an increased susceptibility to breast cancer disease. Our result indicates a potential dominant effect of VEGF (-460T>C) TT genotype on susceptibility to the breast cancer disease.CONCLUSION: VEGF (-460T>C) TT genotype significantly increased the risk of breast cancer. VEGF-A (-460T>C) genetic ariability was significantly associated with distant metastasis of the disease. It may be a useful as predisposing genetic marker for breast cancer .Further studies with larger sample sizes are necessary to confirm our findings..