Interaction of angiogenic microvessels with the extracellular matrix
Interaction of angiogenic microvessels with the extracellular matrix
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DOI：
10.1152/ajpheart.00772.2007
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发表时间：
2007-12-01
影响因子：
4.8
通讯作者：
Weiss, Jeffrey A.
中科院分区：
文献类型：
--
作者：
Krishnan, Laxminarayanan;Hoying, James B.;Weiss, Jeffrey A.
细胞外基质（ECM）通过提供生物化学和位置线索以及通过机械影响微血管细胞行为在血管生成中起关键作用。相当多的信息是已知的有关血管生成的生化线索，但较少知道的机械动力学在活跃的血管生成。本研究的目的是表征血管生成前和血管生成过程中的简单血管生成组织的材料特性的变化。在出芽过程中，组织硬度总体下降，随后在新生血管伸长过程中增加。基质硬度的下降与基质金属蛋白酶mRNA表达峰值和蛋白水解活性升高相一致。ECM组分和细胞-ECM相互作用分子的基因表达升高以及随后的蛋白水解活性下降（尽管酶水平保持升高）与随后的硬化一致。本研究的结果表明，支架组织的机械性能可以在血管生成过程中通过生长的微血管系统进行主动修改。
The extracellular matrix (ECM) plays a critical role in angiogenesis by providing biochemical and positional cues, as well as by mechanically influencing microvessel cell behavior. Considerable information is known concerning the biochemical cues relevant to angiogenesis, but less is known about the mechanical dynamics during active angiogenesis. The objective of this study was to characterize changes in the material properties of a simple angiogenic tissue before and during angiogenesis. During sprouting, there was an overall decrease in tissue stiffness followed by an increase during neovessel elongation. The fall in matrix stiffness coincided with peak matrix metalloproteinase mRNA expression and elevated proteolytic activity. An elevated expression of genes for ECM components and cell-ECM interaction molecules and a subsequent drop in proteolytic activity (although enzyme levels remained elevated) coincided with the subsequent stiffening. The results of this study show that the mechanical properties of a scaffold tissue may be actively modified during angiogenesis by the growing microvasculature.