The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair.
The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair.
复制标题
具有无缝界面层的仿生双相 CAN-PAC 水凝胶的制备应用于骨软骨缺损修复
DOI：
10.1038/boneres.2017.18
复制
发表时间：
2017
期刊：
影响因子：
12.7
通讯作者：
Lin Y
中科院分区：
文献类型：
--
作者：
Liao J;Tian T;Shi S;Xie X;Ma Q;Li G;Lin Y
基于仿生支架的软骨组织工程已成为一种快速发展的软骨缺损修复策略。在本研究中，基于两层之间的密度差，通过热反应，快速交联方法制备了用于骨软骨缺损（OCD）再生的双相CAN-PAC水凝胶。上层水凝胶由CSMA和NIPAm交联，下层水凝胶由PECDA、AAm和PEGDA组成。两层之间的界面首先通过葡萄糖酸钙和海藻酸盐的物理交联接枝，然后通过其他组分的碳-碳双键的化学交联接枝。上层和下层水凝胶的孔径分别为~ 187.4和~ 112.6 μm。上、下水凝胶的模量分别为~ 0.065、~ 0.261 MPa。所制备的双层水凝胶具有模拟成分、模拟结构和模拟刚度的特点，为维持细胞附着和活力提供了微环境。同时，研究了CAN-PAC水凝胶的生物降解性和生物相容性。建立家兔骨软骨缺损模型，将双层水凝胶植入骨软骨缺损。双层水凝胶组再生组织出现新的半透明软骨和修复的软骨下骨，说明水凝胶可以增强骨软骨缺损的修复。
Cartilage tissue engineering based on biomimetic scaffolds has become a rapidly developing strategy for repairing cartilage defects. In this study, a biphasic CAN-PAC hydrogel for osteochondral defect (OCD) regeneration was fabricated based on the density difference between the two layers via a thermally reactive, rapid cross-linking method. The upper hydrogel was cross-linked by CSMA and NIPAm, and the lower hydrogel was composed of PECDA, AAm and PEGDA. The interface between the two layers was first grafted by the physical cross-linking of calcium gluconate and alginate, followed by the chemical cross-linking of the carbon-carbon double bonds in the other components. The pore sizes of the upper and lower hydrogels were~ 187.4 and~ 112.6 μm, respectively. The moduli of the upper and lower hydrogels were~ 0.065 and~ 0.261 MPa. This prepared bilayer hydrogel exhibited the characteristics of mimetic composition, mimetic structure and mimetic stiffness, which provided a microenvironment for sustaining cell attachment and viability. Meanwhile, the biodegradability and biocompatibility of the CAN-PAC hydrogel were examined in vivo. Furthermore, an osteochondral defect model was developed in rabbits, and the bilayer hydrogels were implanted into the defect. The regenerated tissues in the bilayer hydrogel group exhibited new translucent cartilage and repaired subchondral bone, indicating that the hydrogel can enhance the repair of osteochondral defects.