Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules
Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules
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DOI：
10.1016/s1074-7613(00)80160-0
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发表时间：
2000-01-01
期刊：
影响因子：
32.4
通讯作者：
Saeland, S
中科院分区：
文献类型：
--
作者：
Valladeau, J;Ravel, O;Saeland, S
我们已经鉴定出一种II型Ca2+依赖性凝集素，显示甘露糖结合特异性，仅由朗格汉斯细胞（LC）表达，并命名为Langerin。LC的独特特征是伯贝克颗粒（BG），它是由叠加和拉链膜组成的细胞器。在这里，我们已经证明Langerin与BG具有本构性关联，并且Langerin的抗体被内化到这些结构中。值得注意的是，将Langerin cDNA转染成纤维细胞可形成具有典型BG特征的致密膜结构网络。因此，Langerin是一种有效的诱导膜重叠和拉链导致BG的形成。我们的数据表明，诱导BG是Langerin抗原捕获功能的结果，允许进入这些细胞器并提供非经典抗原处理途径。
We have identified a type II Ca2+-dependent lectin displaying mannose-binding specificity, exclusively expressed by Langerhans cells (LC), and named Langerin. LC are uniquely characterized by Birbeck granules (BG), which are organelles consisting of superimposed and zippered membranes. Here, we have shown that Langerin is constitutively associated with BG and that antibody to Langerin is internalized into these structures. Remarkably, transfection of Langerin cDNA into fibroblasts created a compact network of membrane structures with typical features of BG. Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation. Our data suggest that induction of BG is a consequence of the antigen-capture function of Langerin, allowing routing into these organelles and providing access to a nonclassical antigen-processing pathway.