Bystander signal production and response are independent processes which are cell line dependent
Bystander signal production and response are independent processes which are cell line dependent
复制标题
旁观者信号的产生和响应是独立的过程，依赖于细胞系
DOI：
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发表时间：
2008
影响因子：
2.6
通讯作者：
C. Mothersill
中科院分区：
文献类型：
--
作者：
A. Vines;F. Lyng;B. Mcclean;C. Seymour;C. Mothersill
目的：辐射诱导的旁观者效应现在是一种在许多模型中观察到的既定现象;然而，不知道旁观者效应的大小是由受照射细胞产生的信号还是受照射细胞的反应决定的。本研究的目的是确定旁观者信号与旁观者反应在三种不同细胞系模型中的重要性。研究方法：使用细胞系HPV-G、CHO-K1和E89（葡萄糖6-磷酸脱氢酶（G6 DP）无效）建立基质设计实验，以生产辐照细胞条件培养基（ICCM），该培养基用于处理所有细胞系。结果如下：对于HPV-G和CHO-K1细胞系，对自体ICCM的反应与用另一细胞系产生的ICCM处理时的反应显著不同。这些细胞系对E89 ICCM没有反应，E89细胞对任何ICCM也没有显着反应，表明G6 DP在旁观者效应中起关键作用。结论：这些数据表明，至少对于这些细胞系，在能够响应旁观者信号的细胞系的情况下，由照射的细胞产生的信号决定了旁观者效应的大小。
Purpose: Radiation-induced bystander effects are now an established phenomenon seen in numerous models; however it is not known whether the magnitude of the bystander effect is determined by the signal produced by the irradiated cells or the response of the exposed cells. The aim of this investigation is to determine the importance of the bystander signal versus the bystander response in three different cell line models. Methods: A matrix design experiment using cell lines, HPV-G, CHO-K1 and E89 (glucose 6-phosphate dehydrogenase (G6DP) null) was set up to produce irradiated cell conditioned medium (ICCM), which was used to treat all cell lines. Results: For HPV-G and CHO-K1 lines, the response to autologous ICCM was significantly different to that when treated with ICCM generated from another line. These lines displayed no response to E89 ICCM, nor did E89 cells show a significant response to any ICCM, suggesting that G6DP plays a key role in the bystander effect. Conclusion: These data suggest, for these cell lines at least, that in the case of cell lines capable of responding to the bystander signal, it is the signal produced by the irradiated cell that determines the magnitude of the bystander effect.
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影响因子：
11.2
作者：
E. Azzam;S. M. de Toledo;D. Spitz;J. Little
通讯作者：
E. Azzam;S. M. de Toledo;D. Spitz;J. Little
影响因子：
8
作者：
Morgan, WF
通讯作者：
Morgan, WF
DOI：
10.1016/s0027-5107(02)00068-4
发表时间：
2002-06-19
影响因子：
2.3
作者：
Iyer, R;Lehnert, BE
通讯作者：
Lehnert, BE
来自纯突变体菌落的葡萄糖-6-磷酸脱氢酶缺陷型中国仓鼠细胞的分离和表征。
影响因子：
2.7
作者：
Stamato,TD;Richardson,E;Ianacone,J;MacLaren,RA;Denko,N;Giaccia,A
通讯作者：
Giaccia,A