Synergistic control of chloroplast biogenesis by MYB-related and Golden2-like transcription factors
Synergistic control of chloroplast biogenesis by MYB-related and Golden2-like transcription factors
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DOI：
10.1101/2023.08.11.552970
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发表时间：
2023-11
期刊：
影响因子：
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通讯作者：
Eftychios Frangedakis;Nataliya E. Yelina;Kumari Billakurthi;Tina B. Schreier;P. Dickinson;Marta Tomaselli;J. Haseloff;J. Hibberd
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作者：
Eftychios Frangedakis;Nataliya E. Yelina;Kumari Billakurthi;Tina B. Schreier;P. Dickinson;Marta Tomaselli;J. Haseloff;J. Hibberd
叶绿体生物发生依赖于来自GOLDEN 2-LIKE（GLK）转录因子家族的主调节因子，但GLK突变体含有残留叶绿素，因此还必须涉及其他蛋白质。在这里，我们确定MYB相关的转录因子作为调节器的叶绿体生物发生地钱和被子植物拟南芥。在这两个物种中，MYB相关基因的双突变等位基因显示出非常有限的叶绿体发育，光合作用基因表达受到干扰的程度比GLK突变体更大。In M. polymorpha中MYB相关基因作用于GLK的上游，而A. thaliana这种关系已经重新连接。在这两个物种中，编码叶绿素生物合成酶的基因由MYB相关蛋白和GLK蛋白控制，而那些允许CO2固定，光呼吸和光系统组装和修复的基因则需要MYB相关蛋白。因此，MYB相关基因和GLK基因具有重叠以及不同的靶标。我们的结论是，一起MYB相关和GLK转录因子编排叶绿体发育的陆地植物。
Chloroplast biogenesis is dependent on master regulators from the GOLDEN2-LIKE (GLK) family of transcription factors, but glk mutants contain residual chlorophyll and therefore other proteins must also be involved. Here we identify MYB-related transcription factors as regulators of chloroplast biogenesis in the liverwort Marchantia polymorpha and angiosperm Arabidopsis thaliana. In both species, double mutant alleles in MYB-Related genes show very limited chloroplast development, and photosynthesis gene expression is perturbed to a greater extent than in mutants of GLK. In M. polymorpha MYB-related genes act upstream of GLK, while in A. thaliana this relationship has been rewired. In both species, genes encoding enzymes of chlorophyll biosynthesis are controlled by MYB-related and GLK proteins whilst those allowing CO2 fixation, photorespiration and photosystem assembly and repair require the MYB-related proteins. Thus, MYB-related and GLK genes have overlapping as well as distinct targets. We conclude that together MYB-related and GLK transcription factors orchestrate chloroplast development in land plants.