Cell attachment to frozen sections of injured adult mouse brain: effects of tenascin antibody and lectin perturbation of wound-related extracellular matrix molecules.
Cell attachment to frozen sections of injured adult mouse brain: effects of tenascin antibody and lectin perturbation of wound-related extracellular matrix molecules.
复制标题
细胞附着在受伤的成年小鼠大脑冰冻切片上：生腱蛋白抗体和凝集素对伤口相关细胞外基质分子的扰动的影响。
DOI：
10.1016/0165-0270(96)00008-8
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发表时间：
1996
影响因子：
3
通讯作者：
Steindler,DA
中科院分区：
文献类型：
--
作者：
Laywell,ED;Friedman,P;Harrington,K;Robertson,JT;Steindler,DA
Previous studies describing the use of cryoculture methods have focused on the efficacy of the method for studying neuron attachment and neurite outgrowth on intact sections of nerve, and rodent and even human brain. The cryoculture method has shown promise for determining the presence of cell attachment- and neurite-growth-inhibiting molecules in such specimens, and some studies have also attempted to neutralize such molecules with antibodies to myelin inhibitory proteins, nerve growth factor, or factors present in conditioned media that may counteract the repulsiveness of some of these molecules preserved in sections of, for example, myelinated nerves or adult brain white matter. The present study describes the novel use of lesioned central nervous system cryocultures as substrates for investigating the attachment of embryonic neurons and PC12 cells. In addition to demonstrating the use of this novel scar substrate to extend previous ‘scar-in-a-dish’ models (David et al. (1990) Neuron, 5: 463–469; Rudge and Silver (1990) J. Neurosci., 10: 3594–3603; Rudge et al. (1989) Exp. Neurol., 103: 1–16), the present study also describes antibody and lectin perturbations of putative inhibitory molecules that result in an enhanced attachment of cells to cryosection cultures of brain and spinal cord wounds.
