A Multi-Modality CMOS Sensor Array for Cell-Based Assay and Drug Screening
A Multi-Modality CMOS Sensor Array for Cell-Based Assay and Drug Screening
复制标题
DOI：
10.1109/tbcas.2015.2504984
复制
发表时间：
2015-12-01
影响因子：
5.1
通讯作者：
Wang, Hua
中科院分区：
文献类型：
--
作者：
Chi, Taiyun;Park, Jong Seok;Wang, Hua
在本文中，我们提出了一个完全集成的多模态CMOS细胞传感器阵列，具有四种传感模式，以表征不同的细胞生理反应，包括细胞外电压记录，细胞阻抗映射，光学检测与阴影成像和生物发光传感，和热监测。传感器阵列由9个平行的像素组和9个相应的信号调理块组成。每个像素组包括一个温度传感器和16个三模态传感器像素，而每个三模态传感器像素可以独立地配置用于细胞外电压记录、细胞阻抗测量（电压激励/电流感测）和光学检测。该传感器阵列支持像素级的多模态细胞传感，可对同一细胞样本进行整体细胞表征和联合模态生理监测，像素分辨率为80 μ m x 100 μ m。利用不同活细胞样品的综合生物实验证明了所提出的多模态感测在基于细胞的测定和药物筛选中的功能和益处。
In this paper, we present a fully integrated multi-modality CMOS cellular sensor array with four sensing modalities to characterize different cell physiological responses, including extracellular voltage recording, cellular impedance mapping, optical detection with shadow imaging and bioluminescence sensing, and thermal monitoring. The sensor array consists of nine parallel pixel groups and nine corresponding signal conditioning blocks. Each pixel group comprises one temperature sensor and 16 tri-modality sensor pixels, while each tri-modality sensor pixel can be independently configured for extracellular voltage recording, cellular impedance measurement (voltage excitation/current sensing), and optical detection. This sensor array supports multi-modality cellular sensing at the pixel level, which enables holistic cell characterization and joint-modality physiological monitoring on the same cellular sample with a pixel resolution of 80 mu m x 100 mu m. Comprehensive biological experiments with different living cell samples demonstrate the functionality and benefit of the proposed multi-modality sensing in cell-based assay and drug screening.