Renal toxicity after chronic inhalation exposure of rats to trichloroethylene
Renal toxicity after chronic inhalation exposure of rats to trichloroethylene
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DOI：
10.1016/s0378-4274(01)00545-8
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发表时间：
2002-03-10
影响因子：
3.5
通讯作者：
Wilhelm, M
中科院分区：
文献类型：
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作者：
Mensing, T;Welge, P;Wilhelm, M
雄性Long-Evans大鼠暴露于0（对照）或500 ppm三氯乙烯（TRI）6个月，每天6小时，每周5天。测定血中TRI代谢产物三氯乙醇（TCE）和尿中三氯乙酸（TCA）。在尿液中测定与肾损伤相关的特定参数[肾小球损伤的生物标志物：高分子量蛋白（HMW）、白蛋白（ALB）;近端肾小管损伤：N-乙酰-β-D-氨基葡萄糖苷酶（NAG）、低分子量蛋白（LMW）]。尿中NAG和LMW浓度显著升高。使用彗星试验未检测到肾细胞中的DNA链断裂，并进行了组织学检查。在接触大鼠的肾小球和肾小管中观察到组织学改变。肾毒性生物标志物的释放表明，暴露大鼠的近端小管发生了变化。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Male Long-Evans rats were exposed to 0 (controls) or 500 ppm trichloroethylene (TRI) for 6 months, 6 h daily, and 5 days a week. The TRI metabolites trichloroethanol (TCE) in blood and trichloroacetic acid (TCA) in urine were measured. Specific parameters related to the renal damage were determined in urine [biomarker for glomerular damage: high molecular weight proteins (HMW), albumin (ALB); for proximal tubular damage: N-acetyl-beta-D-glucosaminidase (NAG), low-molecular-weight-proteins (LMW)]. Significantly increased concentrations of NAG and LMW in urine of exposed rats were detected. No DNA-strand breaks in kidney cells could be detected using the comet assay, and histological examinations were performed. Histological alterations were observed in glomeruli and tubuli of exposed rats. The release of biomarkers for nephrotoxicity suggested alterations preferably in the proximal tubules of the exposed rats. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.