Peptidomic comparison and characterization of the major components of the venom of the giant ant Dinoponera quadriceps collected in four different areas of Brazil
Peptidomic comparison and characterization of the major components of the venom of the giant ant Dinoponera quadriceps collected in four different areas of Brazil
复制标题
DOI：
10.1016/j.jprot.2013.10.017
复制
发表时间：
2013-12-06
影响因子：
3.3
通讯作者：
Quinton, Loic
中科院分区：
文献类型：
--
作者：
Cologna, Camila Takeno;Cardoso, Jaqueline dos Santos;Quinton, Loic
尽管甲蚁的毒液会造成严重的伤害，但有关其毒液的生物学特性和成分的信息仍然非常有限。Dinoponera属的蚂蚁被认为是世界上最大的蚂蚁，体长3厘米。它们的出现仅限于南美洲的热带地区。在这项工作中，我们研究了毒液的巨型Dinoponera四头蚁收集在4个不同地区的巴西。通过使用互补的质谱方法的组合，我们的目标是：（一）表征这些蚂蚁的毒液组成;（二）建立一个比较分析的毒液从四个不同的地理区域在巴西。这种方法表明蚂蚁毒液是新化合物的丰富来源。鉴定并选择几种肽用于“从头测序”。由于大多数新的肽显示出与抗菌肽（AMP）的相似性，因此进行抗菌测定以评价其活性。在四个地区的比较研究中，我们不仅观察到毒液成分的重大差异，而且在最近的地区收集的毒液比在遥远的地区收集的毒液更相似。这些观察似乎突出了蚂蚁毒液对当地环境的适应。关于生物测定，称为Dq-3162和Da-3177的肽显示出广泛的抗微生物活性。具有广谱活性和不同支架的新AMP的表征可以帮助科学家设计新的治疗剂，并了解这些肽与微生物膜相互作用的机制。本论文首次对蚂蚁毒的蛋白质组学特征进行了全面的描述，并对蚂蚁毒的生物学特性进行了初步的研究。四头肌毒液此外，这项研究报告的原始蚂蚁的毒液成分的变化，从4个不同的地理区域的巴西。结果显示，每个毒液/区域存在类似于335种化合物，并观察到菌落间差异。对16个新的多肽进行了结构鉴定，并对其中2个进行了合成和生物活性测定。这些发现突出了蚂蚁毒液的相当大的和尚未探索的多样性，这些毒液可以在不同的知识领域作为有价值的研究工具。(C)2013爱思唯尔有限公司版权所有。
Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera are believed to be the world's largest living ants with a body length of 3 cm. Their occurrence is restricted to tropical areas of South America. In this work, we study-the venom of the giant Dinoponera quadriceps ant collected in 4 different regions of Brazil. By using a combination of complementary mass spectrometric approaches, we aim at: (i) characterizing the venom composition of these ants; (ii) establishing a comparative analysis of the venom from four geographically different regions in Brazil. This approach demonstrates that ant venom is a copious source of new compounds. Several peptides were identified and selected for "de novo sequencing". Since most of the new peptides showed similarities with antimicrobial peptides (AMPs), antimicrobial assays were performed with the purpose of evaluating their activity. In regard to the comparative study of the four regions, we observed not only major differences in the venom compositions, but also that the venoms collected in closest areas are more similar than the ones collected in distant regions. These observations seem to highlight an adaption of the ant venoms to the local environment. Concerning the biological assays, the peptides called Dq-3162 and Da-3177 showed a wide-ranging antimicrobial activity. The characterization of new AMPs with a broad spectrum of activity and different scaffolds may aid scientists to design new therapeutic agents and understand the mechanisms of those peptides to interact with microbial membranes. The results obtained betoken the biotechnological potential of ant's venom.Biological significanceFor the first time this manuscript describes an extensive proteomics characterization of the D. quadriceps venom. In addition this study reports the variation in venom composition of primitive ants from 4 geographically different areas of Brazil. The results reveal the presence of similar to 335 compounds for each venom/area and inter-colony variations were observed. 16 new peptides were characterized and 2 of them were synthesized and biologically assayed. These findings highlight the considerable and still unexplored diversity of ant's venom which could be used as valuable research tools in different areas of knowledge. (C) 2013 Elsevier B.V. All rights reserved.