Large dense-core vesicle exocytosis in PC12 cells
Large dense-core vesicle exocytosis in PC12 cells
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DOI：
10.1006/meth.1998.0668
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发表时间：
1998-10-01
期刊：
影响因子：
4.8
通讯作者：
Martin, TFJ
中科院分区：
文献类型：
--
作者：
Klenchin, VA;Kowalchyk, JA;Martin, TFJ
调控胞吐的研究目前面临的主要挑战是鉴定介导分泌囊泡通过招募、对接和融合等运输阶段转运的必需蛋白。确定这些必需蛋白质的生理作用和作用机制是至关重要的。无细胞系统中调节胞外分泌阶段的重建提供了鉴定所需蛋白质和建立其阶段特异性作用机制的机会。PC12细胞是肾上腺髓质来源的克隆细胞系，具有大的致密核囊泡，在各种可渗透细胞和分离膜制备中保持其调节胞外分泌的能力。我们描述了几种无细胞系统，用于研究源自PC12细胞的调节胞吐。(C) 1998学术出版社。
A current major challenge in the study of regulated exocytosis is the identification of essential proteins that mediate the transit of secretory vesicles through trafficking stages such as recruitment, docking, and fusion. Defining the physiological roles and mechanisms of action of these essential proteins is paramount. The reconstitution of stages of regulated exocytosis in cell-free systems provides the opportunity to identify required proteins and establish their stage-specific mechanisms of action. PC12 cells, clonal cell lines of adrenal medullary origin, possess large dense-core vesicles that retain their competence for regulated exocytosis in a variety of permeable cell and isolated membrane preparations. We describe several cell-free systems for studies of regulated exocytosis derived from PC12 cells. (C) 1998 Academic Press.