Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary.
Outbreaks of Neuroinvasive Astrovirus Associated with Encephalomyelitis, Weakness, and Paralysis among Weaned Pigs, Hungary.
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DOI：
10.3201/eid2312.170804
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发表时间：
2017-12
影响因子：
11.8
通讯作者：
Reuter G
中科院分区：
文献类型：
--
作者：
Boros Á;Albert M;Pankovics P;Bíró H;Pesavento PA;Phan TG;Delwart E;Reuter G
匈牙利一个大型高产猪场的新断奶猪（25 至 35 日龄）有 2 年神经系统疾病病史，临床症状为后截瘫，死亡率很高。尸检的受影响猪患有脑脊髓炎和神经坏死。通过逆转录 PCR 和原位杂交在该农场 6 只动物的脑和脊髓样本中鉴定出 3 型猪星状病毒。在定量 RT-PCR 检测的组织中，脑干和脊髓中检测到的病毒载量最高。在另外 2 个地理位置相距较远的农场存档的大脑和脊髓样本中也检测到了类似的猪星状病毒 3 型。病毒RNA主要局限于神经元，特别是脑干、小脑（浦肯野细胞）和颈脊髓。星状病毒在粪便中通常检测不到，但存在于呼吸道样本中，表明可能存在呼吸道感染。星状病毒可能引起常见的神经侵袭性流行病。
A large, highly prolific swine farm in Hungary had a 2-year history of neurologic disease among newly weaned (25- to 35-day-old) pigs, with clinical signs of posterior paraplegia and a high mortality rate. Affected pigs that were necropsied had encephalomyelitis and neural necrosis. Porcine astrovirus type 3 was identified by reverse transcription PCR and in situ hybridization in brain and spinal cord samples in 6 animals from this farm. Among tissues tested by quantitative RT-PCR, the highest viral loads were detected in brain stem and spinal cord. Similar porcine astrovirus type 3 was also detected in archived brain and spinal cord samples from another 2 geographically distant farms. Viral RNA was predominantly restricted to neurons, particularly in the brain stem, cerebellum (Purkinje cells), and cervical spinal cord. Astrovirus was generally undetectable in feces but present in respiratory samples, indicating a possible respiratory infection. Astrovirus could cause common, neuroinvasive epidemic disease.