Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappa B-like DNA-binding protein.
Interleukin 1 and cyclic AMP induce kappa immunoglobulin light-chain expression via activation of an NF-kappa B-like DNA-binding protein.
复制标题
白介素 1 和环 AMP 通过激活 NF-kappa B 样 DNA 结合蛋白诱导 kappa 免疫球蛋白轻链表达。
DOI：
10.1128/mcb.9.3.959-964.1989
复制
发表时间：
1989
影响因子：
5.3
通讯作者：
Mizel,SB
中科院分区：
文献类型：
--
作者：
Shirakawa,F;Chedid,M;Suttles,J;Pollok,BA;Mizel,SB
白介素1 （Interleukin 1, IL-1）对κ免疫球蛋白轻链合成和表面免疫球蛋白表达的影响[j] .中华免疫学杂志，32(2):223 - 228,1984)。在本研究中，我们发现IL-1的这些作用被环AMP （cAMP）类似物和cAMP升高药物所模拟。IL-1诱导κ免疫球蛋白轻链基因表达与细胞内cAMP水平升高有关。用IL-1或cAMP孵育70Z/3细胞可激活κ免疫球蛋白增强子，通过在转染了κ增强子-CAT表达质粒的细胞中诱导氯霉素乙酰转移酶（CAT）检测到。相反，缺乏κ免疫球蛋白增强子的CAT质粒在IL-1或cAMP存在时无活性。此外，IL-1和cAMP类似物和诱导剂可诱导NF-κ b样dna结合蛋白的激活，该蛋白对κ免疫球蛋白增强子具有特异性。这些结果表明，cAMP可能作为IL-1的第二信使，通过激活与NF-κB相似或相同的dna结合蛋白，在诱导b前细胞中κ免疫球蛋白轻链合成中发挥重要作用。
Interleukin 1 (IL-1) induces the synthesis of κ immunoglobulin light chains and the expression of surface immunoglobulin in the murine pre-B-cell line 70Z/3 (J. G. Giri, P. W. Kincade, and S. B. Mizel, J. Immunol. 132:223–228, 1984). In the present study, we found that these effects of IL-1 are mimicked by cyclic AMP (cAMP) analogs and cAMP-elevating drugs. The induction of κ immunoglobulin light-chain gene expression by IL-1 was associated with an increase in intracellular cAMP levels. Incubation of 70Z/3 cells with IL-1 or cAMP resulted in the activation of the κ immunoglobulin enhancer, as detected by the induction of chloramphenicol acetyltransferase (CAT) in cells transfected with a κ enhancer-CAT expression plasmid. In contrast, CAT plasmids lacking a κ immunoglobulin enhancer were inactive in the presence of IL-1 or cAMP. Furthermore, IL-1 and cAMP analogs and inducers were found to induce the activation of a NF-κB-like DNA-binding protein that exhibited specificity for the κ immunoglobulin enhancer. These results suggest that cAMP may play an important role as a second messenger for IL-1 in the induction of κ immunoglobulin light-chain synthesis in pre-B cells via the activation of a DNA-binding protein that is similar or identical to NF-κB.