Hedgehog signal expression in articular cartilage of rat temporomandibular joint and association with adjuvant-induced osteoarthritis
Hedgehog signal expression in articular cartilage of rat temporomandibular joint and association with adjuvant-induced osteoarthritis
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大鼠颞下颌关节软骨中刺猬信号的表达及其与佐剂诱导的骨关节炎的关系
DOI：
10.1111/jop.12497
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发表时间：
2016
影响因子：
3.3
通讯作者：
吴慧玲
中科院分区：
文献类型：
--
作者：
徐婷;徐高丽;谷志远;吴慧玲
目的：本研究旨在探讨佐剂诱导的大鼠颞下颌关节骨关节炎（TMJOA）中hedgehog（Hh）表达的变化及其对软骨退变的可能影响。方法：48只雄性Sprague-Dawley大鼠随机分为实验骨关节炎（OA）组和假手术对照组。每组六只大鼠在第 3、7、14 和 28 天时采集双侧颞下颌关节。通过苏木精和伊红、甲苯胺蓝和番红 O 染色评估髁软骨的组织学变化。通过免疫组织化学和蛋白质印迹评估软骨中 Hh 信号相关蛋白的表达，包括 Indian Hedgehog (Ihh)、patched-1 (Ptch1)、smoothened (Smo)、神经胶质瘤相关癌基因同源物 1 (Gli1)。通过Western blot检测软骨中基质金属蛋白酶13（MMP-13）、X型胶原、解整合素和具有血小板反应蛋白基序5的金属蛋白酶5（ADAMTS-5）的蛋白表达。结果：组织学分析显示佐剂诱导的OA组软骨明显退化，包括软骨细胞密度降低、软骨细胞变薄和变薄。 软骨退化，细胞外基质中的蛋白聚糖含量减少。与匹配对照组相比，OA组中Ihh、Ptch1、Smo和Gli1的表达较高，且呈时间依赖性。与匹配的对照大鼠相比，OA软骨中MMP-13、X型胶原和ADAMTS-5的蛋白水平显着增加。结论：这些结果表明Ihh信号的激活可能与佐剂诱导的TMJOA中髁软骨的病理变化相关。
OBJECTIVE:.The aim of this study was to investigate the changes in hedgehog (Hh) expression and its possible effects on cartilage degeneration in adjuvant-induced temporomandibular joint osteoarthritis (TMJOA) of rats..METHODS:.Forty-eight male Sprague-Dawley rats were randomly divided into experimental osteoarthritis (OA) and sham control groups. The bilateral TMJs of six rats from each group were harvested at three, seven, 14, and 28 days. Histological changes in condylar cartilage were assessed by hematoxylin and eosin, toluidine blue, and safranin O staining. The expression of Hh signal-related proteins including Indian hedgehog (Ihh), patched-1 (Ptch1), smoothened (Smo), glioma-associated oncogene homologue1 (Gli1) in cartilage was assessed by immunohistochemistry and Western blot. The protein expression of matrix metalloproteinase-13 (MMP-13), type X collagen, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5) in cartilage was evaluated by Western blot..RESULTS:.The histological analysis showed marked cartilage degeneration in adjuvant-induced OA groups, including reduced cartilage cellular density, thinner and degraded cartilage, and decreased proteoglycan content in the extracellular matrix. Compared with matched control groups, the expression of Ihh, Ptch1, Smo, and Gli1 in the OA groups was higher in a time-dependent manner. The protein levels of MMP-13, type X collagen, and ADAMTS-5 were substantially increased in OA cartilage compared with those in matched control rats..CONCLUSION:.These results indicate that the activation of Ihh signaling may be correlated with pathological changes of condylar cartilage in adjuvant-induced TMJOA.