Mutations affecting the chemosensory neurons of Caenorhabditis elegans.
Mutations affecting the chemosensory neurons of Caenorhabditis elegans.
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DOI：
10.1093/genetics/139.1.171
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发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
T. Starich;R. K. Herman;C. K. Kari;Wen-Hui Yeh;Wendy S. Schackwitz;Marcia W. Schuyler;J. Collet
中科院分区：
文献类型：
--
作者：
T. Starich;R. K. Herman;C. K. Kari;Wen-Hui Yeh;Wendy S. Schackwitz;Marcia W. Schuyler;J. Collet
我们已经确定并表征了95个突变，这些突变减少或消除了两栖类和嗜中性粒细胞神经元的染料填充，并且对生存能力、生育能力或运动几乎没有影响。27个突变自发发生在转座子插入频率高的菌株中。68例在EMS治疗后被隔离。所有的突变都导致一种或多种化学感受性反应的缺陷，例如对氯化铵的趋化性或在饥饿和过度拥挤的条件下形成dauer幼虫。其中75个突变是12个先前定义的基因的等位基因，这些突变先前被证明会导致两栖类超微结构的缺陷。我们已经将20个突变分配给13个新基因，称为dyf-1到dyf-13。我们认为，染料填充缺陷突变体所代表的基因对两栖类和分食虫类化学感受器的分化具有重要意义。
We have identified and characterized 95 mutations that reduce or abolish dye filling of amphid and phasmid neurons and that have little effect on viability, fertility or movement. Twenty-seven mutations occurred spontaneously in strains with a high frequency of transposon insertion. Sixty-eight were isolated after treatment with EMS. All of the mutations result in defects in one or more chemosensory responses, such as chemotaxis to ammonium chloride or formation of dauer larvae under conditions of starvation and overcrowding. Seventy-five of the mutations are alleles of 12 previously defined genes, mutations which were previously shown to lead to defects in amphid ultrastructure. We have assigned 20 mutations to 13 new genes, called dyf-1 through dyf-13. We expect that the genes represented by dye-filing defective mutants are important for the differentiation of amphid and phasmid chemosensilla.