Enhanced Label-Free Nanoplasmonic Cytokine Detection in SARS-CoV-2 Induced Inflammation Using Rationally Designed Peptide Aptamer.
Enhanced Label-Free Nanoplasmonic Cytokine Detection in SARS-CoV-2 Induced Inflammation Using Rationally Designed Peptide Aptamer.
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DOI：
10.1021/acsami.2c14748
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发表时间：
2022-11-02
影响因子：
9.5
通讯作者：
Ding, Feng
中科院分区：
文献类型：
--
作者：
He, Jiacheng;Zhou, Lang;Huang, Gangtong;Shen, Jialiang;Chen, Wu;Wang, Chuanyu;Kim, Albert;Zhang, Zhuoyu;Cheng, Weiqiang;Dai, Siyuan;Chen, Pengyu;Ding, Feng
关键词：
快速而准确的血清细胞因子定量在监测快速演变的感染/炎症性疾病患者的免疫状态方面具有巨大的临床意义，正在进行的SARS-CoV-2大流行就是一个例子。然而，由于目前无标记细胞因子免疫分析的检测范围和检测极限不足，关于用于指导致病性炎症中的靶向免疫途径的预测性细胞因子生物标志物的实时信息严重缺乏。在这项工作中，我们报告了一种高灵敏的局部表面等离子共振成像(LSPRi)免疫分析方法，用于检测无标记白介素6(IL-6)，使用合理设计的多肽适配子作为捕获界面。利用其尺寸小和通过Au-S键在传感表面直接功能化的特点，基于多肽适配子的LSPRi免疫分析方法实现了增强的无标记血清IL-6检测，检测下限降至4.6pg/mL，动态范围宽至~6个数量级(可观察到4.6~1×106pg/mL)。该免疫分析方法已在体外用于SARS-CoV-2诱导的炎症反应的无标记分析，并进一步应用于新冠肺炎患者血清IL-6的快速定量。我们的肽适配子LSPRi免疫分析在无标记细胞因子检测方面显示出巨大的潜力，具有前所未有的传感能力，可以准确和及时地解释炎症状态和疾病进展，并确定预后。
Rapid and precise serum cytokine quantification provides immense clinical significance in monitoring the immune status of patients in rapidly evolving infectious/inflammatory disorders, examplified by the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. However, real-time information on predictive cytokine biomarkers to guide targetable immune pathways in pathogenic inflammation is critically lacking, because of the insufficient detection range and detection limit in current label-free cytokine immunoassays. In this work, we report a highly sensitive localized surface plasmon resonance imaging (LSPRi) immunoassay for label-free Interleukin 6 (IL-6) detection utilizing rationally designed peptide aptamers as the capture interface. Benefiting from its characteristically smaller dimension and direct functionalization on the sensing surface via Au–S bonding, the peptide-aptamer-based LSPRi immunoassay achieved enhanced label-free serum IL-6 detection with a record-breaking limit of detection down to 4.6 pg/mL, and a wide dynamic range of ~6 orders of magnitude (values from 4.6 to 1 × 106 pg/mL were observed). The immunoassay was validated in vitro for label-free analysis of SARS-CoV-2 induced inflammation, and further applied in rapid quantification of serum IL-6 profiles in COVID-19 patients. Our peptide aptamer LSPRi immunoassay demonstrates great potency in label-free cytokine detection with unprecedented sensing capability to provide accurate and timely interpretation of the inflammatory status and disease progression, and determination of prognosis.
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影响因子：
2.4
作者：
Klarenbeek, Alex;Blanchetot, Christophe;Roovers, Rob C.
通讯作者：
Roovers, Rob C.
影响因子：
15
作者：
Krieger, F;Möglich, A;Kiefhaber, T
通讯作者：
Kiefhaber, T
影响因子：
4.1
作者：
Busch, Robert T.;Karim, Farzia;Vasquez, Erick S.
通讯作者：
Vasquez, Erick S.
DOI：
10.1074/jbc.m115.695528
发表时间：
2016-06-24
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blanchetot C;De Jonge N;Desmyter A;Ongenae N;Hofman E;Klarenbeek A;Sadi A;Hultberg A;Kretz-Rommel A;Spinelli S;Loris R;Cambillau C;de Haard H
通讯作者：
de Haard H
影响因子：
82.9
作者：
Del Valle DM;Kim-Schulze S;Huang HH;Beckmann ND;Nirenberg S;Wang B;Lavin Y;Swartz TH;Madduri D;Stock A;Marron TU;Xie H;Patel M;Tuballes K;Van Oekelen O;Rahman A;Kovatch P;Aberg JA;Schadt E;Jagannath S;Mazumdar M;Charney AW;Firpo-Betancourt A;Mendu DR;Jhang J;Reich D;Sigel K;Cordon-Cardo C;Feldmann M;Parekh S;Merad M;Gnjatic S
通讯作者：
Gnjatic S