The development of the pattern of retinal ganglion cells in the chick retina: mechanisms that control differentiation.
The development of the pattern of retinal ganglion cells in the chick retina: mechanisms that control differentiation.
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DOI：
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发表时间：
1999-12
期刊：
影响因子：
4.6
通讯作者：
K. McCabe;E. C. Gunther;T. Reh
中科院分区：
文献类型：
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作者：
K. McCabe;E. C. Gunther;T. Reh
脊椎动物和无脊椎动物眼睛中的神经元都是以规则的阵列组织的。虽然对无脊椎动物眼睛中神经元规则阵列形成的机制了解很多，但对脊椎动物眼睛中神经元镶嵌形成的机制知之甚少。这些研究的目的是确定脊椎动物视网膜中第一个神经元（视网膜神经节细胞）的细胞机制。我们已经发现，小鸡视网膜中的神经节细胞发育为图案阵列，其作为分化的波前从中央向周边视网膜扩散。神经节细胞分化的开始与整体视网膜生长保持同步;然而，在第一节细胞分化的前端没有明确的细胞周期同步。神经节细胞的分化不依赖于来自先前形成的神经节细胞的信号，因为从神经节细胞分化的前部隔离多达400 μ m的外周视网膜并不阻止新的神经节细胞发育。与先前的研究一致，用FGFR的特异性抑制剂阻断FGF受体活化延缓了神经节细胞分化前沿的运动，而外源性FGF 1的应用导致外周视网膜中神经节细胞的早熟发育。我们的观察，与以前的研究一起，支持FGF和FGF受体激活的作用，在视网膜神经节细胞的初始发展从未分化的神经上皮细胞周边的分化扩大波前。
Neurons in both vertebrate and invertebrate eyes are organized in regular arrays. Although much is known about the mechanisms involved in the formation of the regular arrays of neurons found in invertebrate eyes, much less is known about the mechanisms of formation of neuronal mosaics in the vertebrate eye. The purpose of these studies was to determine the cellular mechanisms that pattern the first neurons in vertebrate retina, the retinal ganglion cells. We have found that the ganglion cells in the chick retina develop as a patterned array that spreads from the central to peripheral retina as a wave front of differentiation. The onset of ganglion cell differentiation keeps pace with overall retinal growth; however, there is no clear cell cycle synchronization at the front of differentiation of the first ganglion cells. The differentiation of ganglion cells is not dependent on signals from previously formed ganglion cells, since isolation of the peripheral retina by as much as 400 microm from the front of ganglion cell differentiation does not prevent new ganglion cells from developing. Consistent with previous studies, blocking FGF receptor activation with a specific inhibitor to the FGFRs retards the movement of the front of ganglion cell differentiation, while application of exogenous FGF1 causes the precocious development of ganglion cells in peripheral retina. Our observations, taken together with those of previous studies, support a role for FGFs and FGF receptor activation in the initial development of retinal ganglion cells from the undifferentiated neuroepithelium peripheral to the expanding wave front of differentiation.