Evaluation of effects of shear stress on hepatocytes by a microchip-based system
Evaluation of effects of shear stress on hepatocytes by a microchip-based system
复制标题
DOI：
10.1088/0957-0233/17/12/s08
复制
发表时间：
2006-12
影响因子：
2.4
通讯作者：
Yuki Tanaka;M. Yamato;T. Okano;T. Kitamori;Kiichi Sato
中科院分区：
文献类型：
--
作者：
Yuki Tanaka;M. Yamato;T. Okano;T. Kitamori;Kiichi Sato
开发了一种基于微芯片的剪切应力对肝细胞活性影响的评估系统。剪切流使肝细胞根据其剪应力暴露值而改变形态和功能。高剪切应力下肝细胞代谢活性降低，特别是当培养时间超过3 d时。形态学观察显示肝细胞与血管内皮细胞一样发生形状改变。这表明灌注影响了微芯片中肝细胞的功能和形态。该系统可用于微流控生物反应器的流动条件优化。
A microchip-based evaluation system for the effects of shear stress on hepatocyte activity was developed. The shear flows caused hepatocytes to change morphology and functions according to their exposed value of shear stress. The metabolic activities of hepatocytes under higher shear stress were lower, especially when the cultivation time exceeded 3 days. The morphologic observation revealed that hepatocytes changed their shape like vascular endothelial cells do. This suggested that perfusion affected the function and morphology of hepatocytes in the microchip. This system is useful for the optimization of flow conditions in microfluidic bioreactors.