PHYTOHEMAGGLUTININ STIMULATION OF RAT THYMUS LYMPHOCYTE GLYCOLYSIS
PHYTOHEMAGGLUTININ STIMULATION OF RAT THYMUS LYMPHOCYTE GLYCOLYSIS
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DOI：
10.1016/0304-4165(76)90005-2
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
WEIDEMANN, MJ
中科院分区：
文献类型：
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作者：
CULVENOR, JG;WEIDEMANN, MJ
在加入植物血凝素或刀豆蛋白A 45分钟后，大鼠胸腺细胞的葡萄糖消失和乳酸的产生被显著地刺激，这种刺激速度持续至少8小时。在糖酵解通量增加的非平衡步骤中出现的糖酵解中间产物的稳态浓度的变化表明，葡萄糖载体、己糖激酶和磷酸果糖激酶是潜在的调节步骤，在细胞与有丝分裂原相互作用后经历几乎同时或紧密顺序的激活。
Glucose disappearance and lactate production by rat thymocytes are stimulated significantly 45 min after addition of phytohemagglutinin or concanavalin A; the stimulated rate is sustained for at least 8 h. Changes in the steady-state concentration of glycolytic intermediates that occur at non-equilibrium steps during the increased rate of glycolytic flux indicate that the glucose carrier, hexokinase and phosphofructokinase are potentially regulatory steps that undergo nearly simultaneous or tightly sequential activation following interaction of the cells with the mitogen.