A FAMILY OF CYCLIN HOMOLOGS THAT CONTROL THE G1 PHASE IN YEAST
A FAMILY OF CYCLIN HOMOLOGS THAT CONTROL THE G1 PHASE IN YEAST
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DOI：
10.1073/pnas.86.16.6255
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
REED, SI
中科院分区：
文献类型：
--
作者：
HADWIGER, JA;WITTENBERG, C;REED, SI
两个酿酒酵母基因被分离的基础上，其剂量依赖性的救援的温度敏感性突变的基因CDC 28，它编码的蛋白激酶参与控制细胞分裂。CLN 1和CLN 2编码密切相关的蛋白质，这些蛋白质也与细胞周期蛋白具有同源性。细胞周期蛋白，其特征在于通过细胞周期的丰富的戏剧性的周期性，被认为是参与在动物细胞中的有丝分裂诱导。CLN 2基因中的显性突变CLN 2 -1可促进细胞周期中的G1期向S期的转变，并削弱细胞响应外部信号而停滞在G1期的能力，这表明所编码的蛋白质参与酵母细胞周期的G1期控制。
Two Saccharomyces cerevisiae genes were isolated based upon their dosage-dependent rescue of a temperature-sensitive mutation of the gene CDC28, which encodes a protein-kinase involved in control of cell division. CLN1 and CLN2 encode closely related proteins that also share homology with cyclins. Cyclins, characterized by a dramatic periodicity of abundance through the cell cycle, are thought to be involved in mitotic induction in animal cells. A dominant mutation in the CLN2 gene, CLN2-1, advances the G1- to S-phase transition in cycling cells and impairs the ability of cells to arrest in G1 phase in response to external signals, suggesting that the encoded protein is involved in G1 control of the cell cycle in Saccharomyces.