Mitogen-Activated Protein Kinases 3 and 6 Are Required for Full Priming of Stress Responses in Arabidopsis thaliana
Mitogen-Activated Protein Kinases 3 and 6 Are Required for Full Priming of Stress Responses in Arabidopsis thaliana
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DOI：
10.1105/tpc.108.062158
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发表时间：
2009-03-01
期刊：
影响因子：
11.6
通讯作者：
Conrath, Uwe
中科院分区：
文献类型：
--
作者：
Beckers, Gerold J. M.;Jaskiewicz, Michal;Conrath, Uwe
在植物和动物中，对生物和非生物胁迫的诱导抗性（IR）与引发细胞以更快和更强地激活防御反应有关。据推测，细胞引发涉及潜在信号成分的积累，这些成分直到挑战暴露于压力时才被使用。然而，这种信号传导组分的身份仍然难以捉摸。在这里，我们表明，在拟南芥化学诱导抗性的发展过程中，引发与有丝分裂原活化蛋白激酶（MPK）、MPK 3和MPK 6的mRNA和失活蛋白的积累相关。在挑战暴露于生物或非生物胁迫，这两种酶更强烈地激活引发的植物比在nonprimed植物。这种升高的激活与增强防御基因的表达和IR的发展有关。胁迫诱导的MPK3和MPK6活性的强诱导也见于组成型引发突变体edr1中，而引发缺陷型npr1突变体中的活性减弱。此外，引发的防御基因的表达和IR的mpk3或mpk6突变体中丢失或减少。我们的研究结果表明，信号组分MPK3和MPK6的预应力沉积是引发植物在IR期间完全诱导防御反应的关键步骤。
In plants and animals, induced resistance (IR) to biotic and abiotic stress is associated with priming of cells for faster and stronger activation of defense responses. It has been hypothesized that cell priming involves accumulation of latent signaling components that are not used until challenge exposure to stress. However, the identity of such signaling components has remained elusive. Here, we show that during development of chemically induced resistance in Arabidopsis thaliana, priming is associated with accumulation of mRNA and inactive proteins of mitogen-activated protein kinases (MPKs), MPK3 and MPK6. Upon challenge exposure to biotic or abiotic stress, these two enzymes were more strongly activated in primed plants than in nonprimed plants. This elevated activation was linked to enhanced defense gene expression and development of IR. Strong elicitation of stress-induced MPK3 and MPK6 activity is also seen in the constitutive priming mutant edr1, while activity was attenuated in the priming-deficient npr1 mutant. Moreover, priming of defense gene expression and IR were lost or reduced in mpk3 or mpk6 mutants. Our findings argue that prestress deposition of the signaling components MPK3 and MPK6 is a critical step in priming plants for full induction of defense responses during IR.