Fabrication of highly porous keratin sponges by freeze-drying in the presence of calcium alginate beads
Fabrication of highly porous keratin sponges by freeze-drying in the presence of calcium alginate beads
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DOI：
10.1016/j.msec.2007.11.008
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发表时间：
2008-12-01
期刊：
影响因子：
--
通讯作者：
Tanabe, Toshizumi
中科院分区：
文献类型：
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作者：
Hamasaki, Shinichi;Tachibana, Akira;Tanabe, Toshizumi
将颗粒浸出法和冷冻干燥法相结合，开发了一种新型的高多孔性和柔性角蛋白海绵的制备方法。将还原的角蛋白水溶液与干燥的海藻酸钙珠混合并冻干以得到角蛋白/海藻酸钙复合物，随后用EDTA溶液处理以浸出海藻酸钙珠。所得角蛋白海绵具有足够的柔性，即使在干燥状态下也可以处理，因为其孔隙率非常高（98.9 +/- 0.1%），这是由消除藻酸钙珠和水形成的大孔和小孔产生的。该海绵支持小鼠成纤维细胞的附着和增殖。因此，通过本发明的制造方法得到的角蛋白海绵提供了作为用于组织工程的细胞支架的一种替代方案。(C)2007 Elsevier B. V.保留所有权利。
Novel fabrication method of highly porous and flexible keratin sponges was developed by combining a particulate-leaching method and a freeze-drying method. Reduced keratin aqueous solution was mixed with dried calcium alginate beads and was lyophilized to give keratin/calcium alginate complex, which was subsequently treated with EDTA solution to leach out calcium alginate beads. The resultant keratin sponge was flexible enough to handle even in dried state because of its quite high porosity (98.9 +/- 0.1%), which was brought about by the large and small pores formed by the elimination of calcium alginate beads and water. The sponge supported the attachment and the proliferation of mouse fibroblast cells. Thus, the keratin sponge given by the present fabrication method afforded one alternative as a cell scaffold for tissue engineering. (C) 2007 Elsevier B.V. All rights reserved.