Phosphate dependent, ruthenium red insensitive CA2+ uptake in mung bean mitochondria.
Phosphate dependent, ruthenium red insensitive CA2+ uptake in mung bean mitochondria.
复制标题
绿豆线粒体中磷酸盐依赖性、钌红不敏感的 CA2 摄取。
DOI：
10.1016/0006-291x(83)90686-1
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
A. Moore
中科院分区：
文献类型：
--
作者：
K. Åkerman;A. Moore
研究了绿豆线粒体能量相关的Ca ~（2+）吸收。使用偶氮胂III作为线粒体外Ca 2+的监测器，我们观察到呼吸相关的Ca 2+的摄取，这需要磷酸盐，对钌红不敏感。摄取率的顺序为5纳摩尔/毫克蛋白质/分钟。醋酸盐，硫酸盐和硫代硫酸盐是无法支持钙的摄取。结果表明，虽然植物线粒体积累钙离子的能量依赖的方式，它不是通过一个简单的电泳单端口机制。
Energy linked Ca2+uptake into mung bean mitochondria has been studied. Using arsenazo III as a monitor of extramitochondrial Ca2+, we observe a respiration-linked uptake of Ca2+which requires phosphate and is insensitive to ruthenium red. The rate of uptake is of the order of 5 nmol/mg protein/min. Acetate, sulphate and thiosulphate are unable to support Ca2+uptake. The results suggest that although plant mitochondria accumulate Ca2+in an energy dependent fashion, it is not via a simple electrophoretic uniport mechanism.