Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
Infection and Rapid Transmission of SARS-CoV-2 in Ferrets
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DOI：
10.1016/j.chom.2020.03.023
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发表时间：
2020-05-13
影响因子：
30.3
通讯作者：
Choi, Young Ki
中科院分区：
文献类型：
--
作者：
Kim, Young-Il;Kim, Seong-Gyu;Choi, Young Ki
由严重急性呼吸综合征冠状病毒2（SARS - CoV - 2）引发的2019冠状病毒病（COVID - 19）疫情在中国出现，并迅速在全球传播。为防止SARS - CoV - 2传播，了解SARS - CoV - 2的体内特性是重中之重。我们报告了一种SARS - CoV - 2感染和传播的雪貂模型，该模型重现了人类疾病的某些方面。感染SARS - CoV - 2的雪貂体温升高且病毒发生复制。尽管未观察到死亡情况，但感染SARS - CoV - 2的雪貂在感染后长达8天的时间里，其鼻腔冲洗液、唾液、尿液和粪便中都有病毒排出。在接触后2天，在所有未感染的直接接触雪貂中都检测到了SARS - CoV - 2。此外，一些未感染的间接接触雪貂的病毒RNA呈阳性，这表明存在空气传播。在鼻甲、气管、肺和肠道中检测到病毒抗原，感染的肺中存在急性细支气管炎。因此，雪貂代表了一种COVID - 19的感染和传播动物模型，可能有助于SARS - CoV - 2治疗方法和疫苗的研发。
The outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in China and rapidly spread worldwide. To prevent SARS-CoV-2 dissemination, understanding the in vivo characteristics of SARS-CoV-2 is a high priority. We report a ferret model of SARS-CoV-2 infection and transmission that recapitulates aspects of human disease. SARS-CoV-2-infected ferrets exhibit elevated body temperatures and virus replication. Although fatalities were not observed, SARS-CoV-2-infected ferrets shed virus in nasal washes, saliva, urine, and feces up to 8 days post-infection. At 2 days post-contact, SARS-CoV-2 was detected in all naive direct contact ferrets. Furthermore, a few naive indirect contact ferrets were positive for viral RNA, suggesting airborne transmission. Viral antigens were detected in nasal turbinate, trachea, lungs, and intestine with acute bronchiolitis present in infected lungs. Thus, ferrets represent an infection and transmission animal model of COVID-19 that may facilitate development of SARS-CoV-2 therapeutics and vaccines.