A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions
A virus-induced kidney disease model based on organ-on-a-chip: Pathogenesis exploration of virus-related renal dysfunctions
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基于器官芯片的病毒肾病模型：病毒相关肾功能障碍的发病机制探索
DOI：
10.1016/j.biomaterials.2019.119367
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发表时间：
2019
期刊：
影响因子：
14
通讯作者：
Zhang Zhi-Ling
中科院分区：
文献类型：
--
作者：
Wang Ji;Wang Cheng;Xu Na;Liu Zheng-Fei;Pang Dai-Wen;Zhang Zhi-Ling
肾功能损害常发生于病毒感染，许多病毒特异性感染远端肾小管，但其发病机制尚不清楚。为了探讨病毒相关性肾功能障碍的发病机制，本研究首次建立了猪伪狂犬病病毒（Pseudorabies Virus，PrV）诱导的远端肾小管芯片（distal tubule-on-a-chip，DTC）肾脏病模型。在DTC中成功重建了远端肾小管的屏障结构和钠重吸收。PrV感染后，Na转运蛋白紊乱，重吸收屏障破坏，微绒毛改变，导致Na重吸收的电解质调节功能障碍。并可导致病毒引起的血清电解质异常。这项工作使我们对器官芯片（OOC）在病毒研究中的优势有了新的认识，因为它以其独特的功能复制能力，使我们更好地了解病毒引起的功能障碍的发病机制。
Renal dysfunctions usually happen in viral infections and many viruses specially infect distal renal tubules, however the pathogenesis remains unknown. Here, in order to explore the pathogenesis of virus-related renal dysfunctions, a Pseudorabies Virus (PrV) induced kidney disease model was built on a distal tubule-on-a-chip (DTC), for the first time. The barrier structure and Na reabsorption of distal renal tubules were successfully reconstituted in DTCs. After PrV infection, results showed electrolyte regulation dysfunction in Na reabsorption for the disordered Na transporters, the broken reabsorption barrier, and the transformed microvilli. And it would lead to virus induced serum electrolyte abnormalities. This work brought us a new cognition about the advantages of organ-on-a-chip (OOC) in virus research, for it had given us a better insight into the pathogenesis of virus induced dysfunctions, based on its unique ability in function reproduction.