Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening
Identification of high-molecular-weight proteins with multiple EGF-like motifs by motif-trap screening
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DOI：
10.1006/geno.1998.5341
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发表时间：
1998-07-01
期刊：
影响因子：
4.4
通讯作者：
Ohara, O
中科院分区：
文献类型：
--
作者：
Nakayama, M;Nakajima, D;Ohara, O
为了系统地识别具有EGF样基序的大蛋白质，我们开发了一种计算机辅助的方法，称为基序陷阱筛选。该方法利用了从大小超过5kb的cDNA库中获得的5 '端单程序列数据。使用这种筛选程序，我们能够从8000个冗余人脑cDNA克隆中鉴定出具有多个EGF样基序的蛋白质的5个已知基因和9个新基因。这些新的基因被发现编码一种新的哺乳动物同源果蝇脂肪蛋白，两个七跨膜蛋白含有多个钙粘蛋白和EGF样基序，两个哺乳动物同源果蝇狭缝蛋白，一个身份不明的LDL受体样蛋白，和三个完全未知的蛋白质。蛋白质中的结构域的组织，连同它们的表达谱和精细的染色体位置，已经表明了它们的生物学意义，表明基序陷阱筛选是发现难以通过常规方法鉴定的新基因的有力工具。(C)北京：科学出版社.
To identify large proteins with an EGF-like-motif in a systematic manner, we developed a computer-assisted method called motif-trap screening. The method exploits 5'-end single-pass sequence data obtained from a pool of cDNAs whose sizes exceed 5 kb, Using this screening procedure, we were able to identify five known and nine new genes for proteins with multiple EGF-like-motifs from 8000 redundant human brain cDNA clones. These new genes were found to encode a novel mammalian homologue of Drosophila fat protein, two seven-transmembrane proteins containing multiple cadherin and EGF-like motifs, two mammalian homologues of Drosophila slit protein, an unidentified LDL receptor-like protein, and three totally uncharacterized proteins. The organization of the domains in the proteins, together with their expression profiles and fine chromosomal locations, has indicated their biological significance, demonstrating that motif-trap screening is a powerful tool for the discovery of new genes that have been difficult to identify by conventional methods. (C) 1998 Academic Press.