Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis
Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis
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DOI：
10.1128/aem.02884-07
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发表时间：
2008-08-01
影响因子：
4.4
通讯作者：
Fredricks, David N.
中科院分区：
文献类型：
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作者：
Oakley, Brian B.;Fiedler, Tina L.;Fredricks, David N.
细菌性阴道病(BV)是一种常见的综合征，与妇女的许多不良健康后果有关。尽管BV具有重要的医学价值，但其病原学和微生物生态学仍然知之甚少。我们使用广谱聚合酶链式反应来普查健康和BV感染的阴道微生物生态系统的群落结构，并从这个环境中合成当前公开可用的细菌16S rRNA基因序列数据。BV携带者的阴道细菌群落比非BV携带者的更丰富多样。在97%的序列相似性界限下，28名BV患者的每个患者的可操作分类单元(OTU)的数量几乎是13名非BV患者的三倍：14.8+/-0.7比5.2+/-0.75(平均标准误差)。基于OTU的分析揭示了许多阴道细菌以前隐藏的多样性，这些细菌目前在GenBank中的代表性很低。我们的测序工作产生了许多新的系统类型(我们的123个序列代表了38个以前在阴道生态系统中未发现的OTU)，包括几个新的BV相关OTU，例如属于Prevotea物种复合体的OTU，这些OTU在当前的NCBI数据库中仍然严重不足。在精细的分类尺度上，不同受试者的群落组成差异很大，但在门的水平上，放线杆菌和拟杆菌与BV密切相关。我们的数据描述了阴道生态系统中以前未被认识到的细菌多样性程度。人类阴道中有许多细菌，这些细菌只与已知物种有遥远的亲缘关系，而患有BV的受试者拥有特别丰富的分类群和多样化的细菌群落。
Bacterial vaginosis (BV) is a common syndrome associated with numerous adverse health outcomes in women. Despite its medical importance, the etiology and microbial ecology of BV remain poorly understood. We used broad-range PCR to census the community structure of the healthy and BV-affected vaginal microbial ecosystems and synthesized current publicly available bacterial 16S rRNA gene sequence data from this environment. The community of vaginal bacteria detected in subjects with BV was much more taxon rich and diverse than in subjects without BV. At a 97% sequence similarity cutoff, the number of operational taxonomic units (OTUs) per patient in 28 subjects with BV was nearly three times greater than in 13 subjects without BV: 14.8 +/- 0.7 versus 5.2 +/- 0.75 (mean standard error). OTU-based analyses revealed previously hidden diversity for many vaginal bacteria that are currently poorly represented in GenBank. Our sequencing efforts yielded many novel phylotypes (123 of our sequences represented 38 OTUs not previously found in the vaginal ecosystem), including several novel BV-associated OTUs, such as those belonging to the Prevotella species complex, which remain severely underrepresented in the current NCBI database. Community composition was highly variable among subjects at a fine taxonomic scale, but at the phylum level, Actinobacteria and Bacteroidetes were strongly associated with BV. Our data describe a previously unrecognized extent of bacterial diversity in the vaginal ecosystem. The human vagina hosts many bacteria that are only distantly related to known species, and subjects with BV harbor particularly taxon-rich and diverse bacterial communities.