Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man
Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man
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DOI：
10.1099/ijsem.0.005378
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发表时间：
2022-01-01
影响因子：
2.8
通讯作者：
Kida, Hiroshi
中科院分区：
文献类型：
--
作者：
Abe, Yuko;Fukushima, Kiyoharu;Kida, Hiroshi
从一名老年肺炎患者的痰中分离出一株生长缓慢的分枝杆菌，鉴定为TY59(T)菌株。16S rRNA基因测序表明，该菌株与鸟分枝杆菌复合体成员和密切相关的物种相似。菌株TY59(T)与哥伦比亚分枝杆菌（99.80%）、脆弱分枝杆菌（99.74%）、铁皮分枝杆菌（99.54%）、鸟分枝杆菌亚种的16S rRNA基因序列相似性最高。鸟分枝杆菌（99.54%）和鸟分枝杆菌亚种。silvaticum(99.54%)。内部转录间隔段（ITS）和dna定向RNA聚合酶亚单位β (rpoB)序列的分析结果与16S rRNA基因分析结果相似。与菌株TY59(T)最接近的种是哥伦比亚分枝杆菌和脆弱分枝杆菌，ITS和rpoB的同源性分别为97.90 ~ 98.25%和96.4 ~ 96.6%。该菌株的65 kDa热休克蛋白（hsp65）基因与脆弱支原体、哥伦比亚支原体和禽支原体不同。具有72.4- 74.2%的同源性。平均核苷酸鉴定结果显示，与M. vulnerability的匹配度最高为93.4%。表型上，无显色性，菌落粗糙，在42℃下生长，硝酸盐还原，β -葡萄糖苷酶和Tween 80水解阴性结果，过氧化氢酶活性阳性结果使该菌株与密切相关的物种区别开。我们认为senriense分枝杆菌是一种生长缓慢的分枝杆菌。型应变为TY59(T) (RIMD 1371001（T)=CIP 111917(T)）。
A slowly growing mycobacteria, identified as strain TY59(T), was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the Mycobacterium avium complex and closely related species. Strain TY59(T) has highest 16S rRNA gene sequence similarities to the type strains of Mycobacterium colombiense (99.80% sequence similarity), Mycobacterium vulneris (99.74 %), Mycobacterium timonense (99.54%), Mycobacterium avium subsp. avium (99.54%) and Mycobacterium avium subsp. silvaticum (99.54%). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta (rpoB) sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59(T) were M. colombiense and M. vulneris with 97.90-98.25 % identity in ITS and 96.4-96.6% in rpoB. The strain's 65 kDa heat shock protein (hsp65) gene was different from those of M. vulneris, M. colombiense and M. avium subsp. silvaticum with 72.4-74.2 % identity. Average nucleotide identity results showed a 93.4 % match to M. vulneris as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 degrees C, negative results for nitrate reduction, beta-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that Mycobacterium senriense sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59(T) (RIMD 1371001(T)=CIP 111917(T)).