Dentin sialoprotein, dentin phosphoprotein, enamelysin and ameloblastin:: tooth-specific molecules that are distinctively expressed during murine dental differentiation
Dentin sialoprotein, dentin phosphoprotein, enamelysin and ameloblastin:: tooth-specific molecules that are distinctively expressed during murine dental differentiation
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DOI：
10.1046/j.0909-8836.1998.eos106510.x
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发表时间：
1998-10-01
影响因子：
1.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Bègue-Kirn, C;Krebsbach, PH;Butler, WT
牙本质涎磷蛋白[命名为DSPP，切割成牙本质涎蛋白（DSP）和牙本质磷蛋白（DPP）I，釉质溶解素和成釉蛋白在牙齿发育过程中以独特的方式表达。这些成分可能参与细胞分化和未矿化基质向矿化结构的转化。为了描绘这三种分子在生理环境中的时间和定位，我们通过在发育中的小鼠组织中的连续切片上进行原位杂交实验来比较它们的表达谱。DSPP mRNA在成牙本质细胞和成釉细胞中，成牙本质细胞和成釉细胞层中的釉质溶素mRNA，成釉细胞前体、极化成牙本质细胞和成釉细胞中的成釉蛋白mRNA的杂交信号被独特地检测到。免疫组化显示DSP和成釉蛋白转录物被翻译成沉积在分化细胞（分别为成牙本质细胞和成釉细胞）的顶端的蛋白质。这些牙齿特异性分子的相关表达谱说明了特定分子网络在启动高度调控的过程（如细胞分化和随后的矿化）中的重要性。
Dentin sialophosphoprotein [designated DSPP and cleaved into dentin sialoprotein (DSP) and dentin phosphoprotein (DPP)I, enamelysin and ameloblastin are each expressed in unique fashions during tooth development. It is possible that these components participate in cell differentiation and the conversion of unmineralized matrix into mineralized structures. In order to delineate the timing and the positioning of these three molecules in a physiological context, we compared their expression profiles by performing in situ hybridization experiments on consecutive sections in developing mouse tissues. Hybridization signals were uniquely detected for DSPP mRNA in odontoblasts and preameloblasts, for enamelysin mRNA in odontoblasts and in the facing ameloblast layer, and for ameloblastin mRNA in preodontoblasts, polarizing odontoblasts and ameloblasts. Immunohistochemistry showed that DSP and ameloblastin transcripts were translated into proteins that were deposited at the apical pole of the differentiated cells (odontoblasts and ameloblasts, respectively). The interrelated expression profiles found for these tooth-specific molecules illustrate the importance of a specific molecular network to initiate highly regulated processes such as cytodifferentiation and the subsequent mineralization.