Iontophoresis and sonophoresis stimulate epidermal cytokine expression at energies that do not provoke a barrier abnormality: Lamellar body secretion and cytokine expression are linked to altered epidermal calcium levels
Iontophoresis and sonophoresis stimulate epidermal cytokine expression at energies that do not provoke a barrier abnormality: Lamellar body secretion and cytokine expression are linked to altered epidermal calcium levels
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DOI：
10.1046/j.1523-1747.2003.12566.x
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发表时间：
2003-11-01
影响因子：
6.5
通讯作者：
Lee, SH
中科院分区：
文献类型：
--
作者：
Choi, EH;Kim, MJ;Lee, SH
我们进行这项研究是为了确定表皮细胞因子的表达是否会因表皮钙含量的变化而改变，而独立于皮肤屏障的破坏。在无毛小鼠皮肤上施加离子电泳和声电泳，其能量不破坏皮肤屏障，但会引起表皮钙梯度的变化。在离子导入和声导入之后，立即在含钙溶液中浸泡，然后在高钙或低钙溶液中进行离子导入。取活检标本进行实时定量RT-PCR检测表皮中白细胞介素-1 α (il -1 α)、肿瘤坏死因子- α (tnf - α)和转化生长因子- β mRNA水平的变化，并用il -1 α和tnf - α一抗进行免疫组化染色。与未处理的对照组相比，经离子导入和声导入处理的表皮以及作为阳性对照的胶带剥离皮肤中，每种细胞因子mRNA的表达都有所增加，而浸泡在高钙溶液中比浸泡在低钙溶液中表达更低。低钙离子导入后il -1 α和tnf - α免疫组化蛋白染色升高。这些研究表明，在体内，表皮钙的变化可以直接指示表皮细胞因子的表达，而不依赖于屏障功能的变化。
We performed this study to identify whether the expression of epidermal cytokines is altered by changes in epidermal calcium content, independent of skin barrier disruption. Iontophoresis and sonophoresis with the energies that do not disrupt the skin barrier, but induce changes in the epidermal calcium gradient, were applied to the skin of hairless mice. Immediately after iontophoresis and sonophoresis, immersion in a solution containing calcium was carried out, and iontophoresis in either high- or low-calcium solutions was performed. The biopsy specimens were taken for real-time quantitative RT-PCR to detect changes in mRNA level of interleukin-1alpha (IL-1alpha), tumor necrosis factor-alpha (TNF-alpha), and transforming growth factor-beta in the epidermis and for immunohistochemical stain with primary antibodies to IL-1alpha and TNF-alpha. The expression of each cytokine mRNA increased in the epidermis treated with iontophoresis and sonophoresis compared to a nontreated control as well as in tape-stripped skin used as a positive control and was lower after immersion in a high-calcium solution than in low-calcium solution. IL-1alpha and TNF-alpha immunohistochemical protein staining increased with iontophoresis at low calcium. These studies suggest that changes in epidermal calcium can directly signal expression of epidermal cytokines in vivo, independent of changes in barrier function.