Carrageenan as an adjuvant to enhance peptide-based vaccine potency.
Carrageenan as an adjuvant to enhance peptide-based vaccine potency.
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DOI：
10.1016/j.vaccine.2010.05.068
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发表时间：
2010-07-19
期刊：
影响因子：
5.5
通讯作者：
Wu, T. -C.
中科院分区：
文献类型：
--
作者：
Zhang, Yu-Qian;Tsai, Ya-Chea;Monie, Archana;Hung, Chien-Fu;Wu, T. -C.
迫切需要新的创新疗法，以对抗与晚期癌症相关的高死亡率和高发病率。基于多肽疫苗的抗原特异性癌症免疫治疗因其简单易行而成为控制癌症的一种有吸引力的方法。然而，这种方法需要使用适当的佐剂。在本研究中，我们探索了红藻多糖化合物卡拉胶(CGN)作为佐剂在HPV-16 E7多肽疫苗免疫小鼠中产生抗原特异性免疫应答和抗肿瘤效应的作用。我们发现角叉菜胶能通过TLR4激活途径显著增强E7多肽疫苗产生的E7特异性免疫应答。此外，角叉菜胶能增强E7多肽疫苗对E7表达肿瘤的保护和治疗作用。此外，观察到的增强不仅限于E7抗原，而且也适用于其他抗原系统。我们还发现，其他与CGN结构相似的化合物，如葡聚糖，也有类似的免疫增强作用。因此，我们的数据表明，CGN及其结构相关的化合物可能成为增强多肽疫苗效力的创新佐剂。
New innovative therapies are urgently required in order to combat the high mortality and morbidity associated with advanced cancers. Antigen-specific cancer immunotherapy using peptide-based vaccination has emerged as an attractive approach for the control of cancers due to its simplicity and easy preparation. However, such an approach requires the employment of suitable adjuvants. In the current study, we explored the employment of a sulfated polysaccharide compound from red algae, carrageeenan (CGN) as an adjuvant for their ability to generate antigen-specific immune responses and antitumor effects in mice vaccinated with human papillomavirus type 16 (HPV-16) E7 peptide vaccine. We found that carrageenan can significantly enhance the E7-specific immune responses generated by E7 peptide vaccination via the TLR4 activation pathway. In addition, carrageenan could enhance the protective and therapeutic antitumor effects generated by E7 peptide vaccination against E7-expressing tumors. Furthermore, the observed enhancement was not restricted to E7 antigen but was also applicable to other antigenic systems. We also found that other structurally similar compounds to CGN, such as dextran, also generated similar immune enhancement. Thus, our data suggest that CGN and its structurally related compounds may serve as innovative adjuvants for enhancing peptide-based vaccine potency.
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