In situ detection of tissue factor in vascular endothelial cells: Correlation with the malignant phenotype of human breast disease
In situ detection of tissue factor in vascular endothelial cells: Correlation with the malignant phenotype of human breast disease
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DOI：
10.1038/nm0296-209
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发表时间：
1996-02-01
期刊：
影响因子：
82.9
通讯作者：
Rickles, FR
中科院分区：
文献类型：
--
作者：
Contrino, J;Hair, G;Rickles, FR
在内皮细胞中组织因子 (TF) 的表达在人类疾病中很少观察到，并且被认为是在体外作为组织培养的产物在血管内皮细胞 (VEC) 表面上精细表达的。使用单克隆抗体和功能性 TF 的新型探针，我们将 TF 定位于 7 名浸润性乳腺癌患者肿瘤内的 VEC（和肿瘤细胞），但未定位于 10 名乳腺纤维囊性病患者良性肿瘤的 VEC（或肿瘤细胞）。强效促凝血 TF 被证明是人类乳腺癌血管生成起始的标志物。进一步的证据表明，TF 证明交联纤维蛋白仅在恶性肿瘤的 VEC 中具有类似的分布。我们将这些数据解释为进一步支持肿瘤细胞可以激活附近的 VEC 并调节体内血管生长的概念。需要进行大型临床病理学研究来确定 TF 是否是人类乳腺疾病中“转变为血管生成表型”的有用标志物和/或与乳腺癌的血栓栓塞并发症相关。
Expression of tissue factor (TF) in the endothelium has been observed only rarely in human disease and has been thought to be elaborated on the surface of vascular endothelial cells (VECs) in vitro as an artifact of tissue culture. Using monoclonal antibodies and a novel probe for functional TF, we have localized TF to the VECs (and tumor cells) within the tumors of seven patients with invasive breast cancer but not in the VECs (or tumor cells) of benign tumors from ten patients with fibrocystic disease of the breast. The potent procoagulant TF was shown to be a marker of the initiation of angiogenesis in human breast cancer. Further evidence that the TF was the demonstration of a similar distribution of cross-linked fibrin only in the VECs of the malignant tumors. We interpret these data as further support for the concept that tumor cells can activate nearby VECs and regulate blood vessel growth in vivo. Large clinical-pathologic studies will be necessary to determine whether TF is a useful marker for the ''switch to the angiogenic phenotype'' in human breast disease and/or correlates with the thromboembolic complications of breast cancer.