Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells.
Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells.
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II型胶原蛋白在退变髓核中下调并导致人髓核细胞变性和凋亡
DOI:
10.3892/mmr.2017.7178
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发表时间:
2017-10
影响因子:
3.4
通讯作者:
Huang D
中科院分区:
文献类型:
--
作者:
Lian C;Gao B;Wu Z;Qiu X;Peng Y;Liang A;Xu C;Su P;Huang D
Degenerative disc disease (DDD) is a common degenerative condition initiated mainly within the nucleus pulposus (NP). To date, the etiopathogenesis of DDD remains unclear, and because no effective therapeutic strategies are available to target its pathological processes, DDD is still treated with symptomatic interventions that are far from adequate. Collagen type II is one of the major matrix components of the NP, and is considered to be essential to NP homeostasis. However, the specific mechanisms by which collagen type II influences NP cells remain unknown. In the present study, collagen type II expression was detected using immunohistochemistry analysis and quantitative polymerase chain reaction, and it was demonstrated to be significantly downregulated in NP tissues from patients with DDD compared with nondegenerative controls. To further explore the mechanism in vitro, interleukin (IL)-1β stimulation was used to induce degeneration of a human NP cell line. IL-1β stimulation upregulated both the mRNA and protein levels of the catabolic markers matrix metalloproteinase 13 (MMP13) and a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4), while it downregulated the anabolic makers aggrecan and collagen type II. However, addition of purified collagen type II prevented this IL-1β-induced metabolic disturbance of the NP cells. Furthermore, IL-1β stimulation significantly promoted apoptosis in NP cells, while collagen type II treatment decreased the apoptotic rate and the protein levels of cleaved caspase-3. In conclusion, collagen type II exhibited protective effects in suppressing NP cell degeneration through its anticatabolic, proanabolic and antiapoptotic effects, suggesting that it may be a promising therapeutic agent for the prevention and treatment of DDD.
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影响因子:
5.6
作者:
Zhou X;Tao Y;Liang C;Zhang Y;Li H;Chen Q
通讯作者:
Chen Q
DOI:
10.1016/s0140-6736(12)61729-2
发表时间:
2012-12-15
期刊:
Lancet (London, England)
影响因子:
--
作者:
Vos T;Flaxman AD;Naghavi M;Lozano R;Michaud C;Ezzati M;Shibuya K;Salomon JA;Abdalla S;Aboyans V;Abraham J;Ackerman I;Aggarwal R;Ahn SY;Ali MK;Alvarado M;Anderson HR;Anderson LM;Andrews KG;Atkinson C;Baddour LM;Bahalim AN;Barker-Collo S;Barrero LH;Bartels DH;Basáñez MG;Baxter A;Bell ML;Benjamin EJ;Bennett D;Bernabé E;Bhalla K;Bhandari B;Bikbov B;Bin Abdulhak A;Birbeck G;Black JA;Blencowe H;Blore JD;Blyth F;Bolliger I;Bonaventure A;Boufous S;Bourne R;Boussinesq M;Braithwaite T;Brayne C;Bridgett L;Brooker S;Brooks P;Brugha TS;Bryan-Hancock C;Bucello C;Buchbinder R;Buckle G;Budke CM;Burch M;Burney P;Burstein R;Calabria B;Campbell B;Canter CE;Carabin H;Carapetis J;Carmona L;Cella C;Charlson F;Chen H;Cheng AT;Chou D;Chugh SS;Coffeng LE;Colan SD;Colquhoun S;Colson KE;Condon J;Connor MD;Cooper LT;Corriere M;Cortinovis M;de Vaccaro KC;Couser W;Cowie BC;Criqui MH;Cross M;Dabhadkar KC;Dahiya M;Dahodwala N;Damsere-Derry J;Danaei G;Davis A;De Leo D;Degenhardt L;Dellavalle R;Delossantos A;Denenberg J;Derrett S;Des Jarlais DC;Dharmaratne SD;Dherani M;Diaz-Torne C;Dolk H;Dorsey ER;Driscoll T;Duber H;Ebel B;Edmond K;Elbaz A;Ali SE;Erskine H;Erwin PJ;Espindola P;Ewoigbokhan SE;Farzadfar F;Feigin V;Felson DT;Ferrari A;Ferri CP;Fèvre EM;Finucane MM;Flaxman S;Flood L;Foreman K;Forouzanfar MH;Fowkes FG;Franklin R;Fransen M;Freeman MK;Gabbe BJ;Gabriel SE;Gakidou E;Ganatra HA;Garcia B;Gaspari F;Gillum RF;Gmel G;Gosselin R;Grainger R;Groeger J;Guillemin F;Gunnell D;Gupta R;Haagsma J;Hagan H;Halasa YA;Hall W;Haring D;Haro JM;Harrison JE;Havmoeller R;Hay RJ;Higashi H;Hill C;Hoen B;Hoffman H;Hotez PJ;Hoy D;Huang JJ;Ibeanusi SE;Jacobsen KH;James SL;Jarvis D;Jasrasaria R;Jayaraman S;Johns N;Jonas JB;Karthikeyan G;Kassebaum N;Kawakami N;Keren A;Khoo JP;King CH;Knowlton LM;Kobusingye O;Koranteng A;Krishnamurthi R;Lalloo R;Laslett LL;Lathlean T;Leasher JL;Lee YY;Leigh J;Lim SS;Limb E;Lin JK;Lipnick M;Lipshultz SE;Liu W;Loane M;Ohno SL;Lyons R;Ma J;Mabweijano J;MacIntyre MF;Malekzadeh R;Mallinger L;Manivannan S;Marcenes W;March L;Margolis DJ;Marks GB;Marks R;Matsumori A;Matzopoulos R;Mayosi BM;McAnulty JH;McDermott MM;McGill N;McGrath J;Medina-Mora ME;Meltzer M;Mensah GA;Merriman TR;Meyer AC;Miglioli V;Miller M;Miller TR;Mitchell PB;Mocumbi AO;Moffitt TE;Mokdad AA;Monasta L;Montico M;Moradi-Lakeh M;Moran A;Morawska L;Mori R;Murdoch ME;Mwaniki MK;Naidoo K;Nair MN;Naldi L;Narayan KM;Nelson PK;Nelson RG;Nevitt MC;Newton CR;Nolte S;Norman P;Norman R;O'Donnell M;O'Hanlon S;Olives C;Omer SB;Ortblad K;Osborne R;Ozgediz D;Page A;Pahari B;Pandian JD;Rivero AP;Patten SB;Pearce N;Padilla RP;Perez-Ruiz F;Perico N;Pesudovs K;Phillips D;Phillips MR;Pierce K;Pion S;Polanczyk GV;Polinder S;Pope CA 3rd;Popova S;Porrini E;Pourmalek F;Prince M;Pullan RL;Ramaiah KD;Ranganathan D;Razavi H;Regan M;Rehm JT;Rein DB;Remuzzi G;Richardson K;Rivara FP;Roberts T;Robinson C;De Leòn FR;Ronfani L;Room R;Rosenfeld LC;Rushton L;Sacco RL;Saha S;Sampson U;Sanchez-Riera L;Sanman E;Schwebel DC;Scott JG;Segui-Gomez M;Shahraz S;Shepard DS;Shin H;Shivakoti R;Singh D;Singh GM;Singh JA;Singleton J;Sleet DA;Sliwa K;Smith E;Smith JL;Stapelberg NJ;Steer A;Steiner T;Stolk WA;Stovner LJ;Sudfeld C;Syed S;Tamburlini G;Tavakkoli M;Taylor HR;Taylor JA;Taylor WJ;Thomas B;Thomson WM;Thurston GD;Tleyjeh IM;Tonelli M;Towbin JA;Truelsen T;Tsilimbaris MK;Ubeda C;Undurraga EA;van der Werf MJ;van Os J;Vavilala MS;Venketasubramanian N;Wang M;Wang W;Watt K;Weatherall DJ;Weinstock MA;Weintraub R;Weisskopf MG;Weissman MM;White RA;Whiteford H;Wiersma ST;Wilkinson JD;Williams HC;Williams SR;Witt E;Wolfe F;Woolf AD;Wulf S;Yeh PH;Zaidi AK;Zheng ZJ;Zonies D;Lopez AD;Murray CJ;AlMazroa MA;Memish ZA
通讯作者:
Memish ZA
DOI:
10.1016/j.spinee.2012.02.027
发表时间:
2013-03
期刊:
The spine journal : official journal of the North American Spine Society
影响因子:
--
作者:
Vo NV;Hartman RA;Yurube T;Jacobs LJ;Sowa GA;Kang JD
通讯作者:
Kang JD
影响因子:
4.6
作者:
Jiang W;Zhang X;Hao J;Shen J;Fang J;Dong W;Wang D;Zhang X;Shui W;Luo Y;Lin L;Qiu Q;Liu B;Hu Z
通讯作者:
Hu Z
影响因子:
--
作者:
Hoy, Damian;Bain, Christopher;Buchbinder, Rachelle
通讯作者:
Buchbinder, Rachelle