DEFICIENT CELLULAR-IMMUNITY IN ENDOMETRIOSIS
DEFICIENT CELLULAR-IMMUNITY IN ENDOMETRIOSIS
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DOI：
10.1016/0002-9378(81)90598-6
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发表时间：
1981-01-01
影响因子：
9.8
通讯作者：
BAKER, GF
中科院分区：
文献类型：
--
作者：
DMOWSKI, WP;STEELE, RW;BAKER, GF
采用体内和体外实验方法，对5例自发性子宫内膜异位症恒河猴的细胞介导的自身免疫反应进行了研究。并与5只正常恒河猴进行比较。体内研究包括皮内注射从子宫内膜、异位内膜或腹膜制备的自体抗原，或注射有丝分裂原植物血凝素（PHA）。在48小时时获得皮肤活检，并在光学显微镜下评价血管周围淋巴细胞浸润。在注射自体子宫内膜抗原后，子宫内膜异位症动物中每个高倍视野的淋巴细胞平均数显著低于对照组。两组间对自体腹膜抗原的淋巴细胞反应无显著差异。两组动物均对PHA反应迅速，血管周围淋巴细胞浸润明显。淋巴细胞刺激反应相同的自体抗原或PHA进行了评价，通过在体外测定和微量的方法。子宫内膜异位症组淋巴细胞对自体子宫内膜抗原的刺激反应明显低于对照组。两组间对腹膜抗原的反应无显著差异;两组所有动物对PHA的反应均显著。患有自发性子宫内膜异位症的恒河猴对自体抗原的细胞免疫反应明显改变。虽然动物的免疫能力，判断PHA的反应，在体内和体外研究表明，减少自体子宫内膜抗原的细胞介导的免疫反应。从正常位置移位的子宫内膜细胞可能仅在细胞介导的免疫特异性改变的妇女中植入。子宫内膜异位症的病因有哪些？
Cell-mediated autoimmune responses were studied by in vivo and in vitro techniques in 5 rhesus monkeys with spontaneous endometriosis. The results were compared with similar data obtained from 5 normal rhesus monkeys without endometriosis. The in vivo studies included intradermal injection of autologous antigens prepared from uterine endometrium, ectopic endometrium or peritoneum, or injection of the mitogen, phytohemagglutinin (PHA). Skin biopsies were obtained at 48 h and evaluated under the light microscope for perivascular lymphocytic infiltration. The mean number of lymphocytes per high-power field after injection of the autologous endometrial antigen was significantly smaller in animals affected by endometriosis than in the control group. There was no significant difference in lymphocytic response to autologous peritoneal antigens between the groups. Animals of both groups responded readily to PHA with marked perivascular lymphocytic infiltration. Lymphocyte stimulation responses to the same autologous antigens or to PHA were evaluated by means of in vitro assays and micro-methods. Lymphocyte stimulation response to autologous endometrial antigens in the group with endometriosis was significantly less than in the control group. There was no significant difference in response to the peritoneal antigens between the groups; the response to PHA was marked in all animals of both groups. Rhesus monkeys with spontaneous endometriosis apparently have an altered cellular immune response to autologous antigens. Although the animals were immunologically competent, as judged by responses to PHA, in vivo and in vitro studies indicated a decrease in the cell-mediated immune response to autologous endometrial antigens. Endometrial cells translocated from their normal location may implant only in women with specific alteration in cell-mediated immunity. New light is thus shed on the cause of endometriosis.