Immortal time: Circadian clock properties of rat suprachiasmatic cell lines
Immortal time: Circadian clock properties of rat suprachiasmatic cell lines
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DOI：
10.1126/science.283.5402.693
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发表时间：
1999-01-29
期刊：
影响因子：
56.9
通讯作者：
Cassone, VM
中科院分区：
文献类型：
--
作者：
Earnest, DJ;Liang, FQ;Cassone, VM
对源自大鼠视交叉上核 (SCN) 的细胞系进行原位筛选，以了解 SCN 独特的生物钟特性。永生化的 SCN 细胞在代谢标记物 2-脱氧葡萄糖的摄取及其神经营养素的含量方面产生了强有力的节律。体外这些节律之间的相位关系与体内 SCN 所表现出的相位关系相同。移植 SCN 细胞系（而非中脑细胞系或成纤维细胞系）恢复了心律失常、SCN 损伤的大鼠的昼夜节律活动。因此，永生化的 SCN 祖细胞不仅保留了 SCN 独特的振荡器、起搏器和时钟特性，而且还维持了适当的昼夜节律相位关系。
Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins. The phase relationship between these rhythms in vitro was identical to that exhibited by the SCN in vivo. Transplantation of SCN cell lines, but not mesencephalic or fibroblast Lines, restored the circadian activity rhythm in arrhythmic, SCN-lesioned rats. Thus, distinctive oscillator, pacemaker, and clock properties of the SCN are not only retained but also maintained in an appropriate circadian phase relationship by immortalized SCN progenitors.