RELATIONSHIP BETWEEN THE COENZYME-A AND THE CARNITINE POOLS IN HUMAN SKELETAL-MUSCLE AT REST AND AFTER EXHAUSTIVE EXERCISE UNDER NORMOXIC AND ACUTELY HYPOXIC CONDITIONS
RELATIONSHIP BETWEEN THE COENZYME-A AND THE CARNITINE POOLS IN HUMAN SKELETAL-MUSCLE AT REST AND AFTER EXHAUSTIVE EXERCISE UNDER NORMOXIC AND ACUTELY HYPOXIC CONDITIONS
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DOI：
10.1172/jci117488
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发表时间：
1994-10-01
影响因子：
15.9
通讯作者：
KRAHENBUHL, S
中科院分区：
文献类型：
--
作者：
FRIOLET, R;HOPPELER, H;KRAHENBUHL, S
研究人员对 6 名人类志愿者在常氧和低氧条件下休息时和力竭运动后的骨骼肌 CoA 和肉碱代谢进行了研究。与休息时的值相比，在常氧和低氧条件下，力竭运动与骨骼肌乳酸增加三到四倍，乙酰辅酶A含量增加两倍相关。由于运动不会显着影响骨骼肌 CoA 自由基 (CoASH)、总酸溶性或总 CoA 含量，因此乙酰辅酶 A 含量的增加是以不同于乙酰辅酶 A 的短链酰基辅酶 A 为代价的。在进行力竭运动时，在常氧和缺氧条件下，骨骼肌乙酰肉碱和短链酰基肉碱含量均增加三到四倍。与 CoA 池相反，这些增加与游离肉碱含量的减少有关，而总酸溶性肉碱和总肉碱含量不受运动影响。力竭运动后，骨骼肌乙酰辅酶A/CoASH比值与相应的乙酰肉碱/游离肉碱比值呈线性相关。运动期间血浆短链酰基肉碱浓度增加了两到三倍，并且与运动完成后40分钟静息时的值没有显着差异。因此，目前的研究说明了人类骨骼肌运动中 CoA 和肉碱池之间的密切相互作用，并强调了肉碱在力竭运动期间维持肌肉 CoASH 含量的重要作用。
Skeletal muscle CoA and carnitine metabolism were investigated in six human volunteers at rest and after exhaustive exercise under normoxic and hypoxic conditions. In comparison to the values at rest, exhaustive exercise was associated with a three- to fourfold increase in the skeletal muscle lactate, and with a twofold increase in the acetyl-CoA content, both under normoxic and hypoxic conditions. Since exercise did not significantly affect the skeletal muscle CoA radical (CoASH), total acid-soluble, or total CoA contents, the increase in the acetyl-CoA content was at the expense of short-chain acyl-CoAs different from acetyl-CoA. With exhaustive exercise, the skeletal muscle acetylcarnitine and short-chain acylcarnitine contents increased by a factor of three to four both under normoxic and hypoxic conditions. In contrast to the CoA pool, these increases were associated with a decrease in the free carnitine content, whereas the total acid-soluble and total carnitine contents were not affected by exercise. After exhaustive exercise, the skeletal muscle acetyl-CoA/CoASH ratio showed a Linear correlation with the corresponding acetylcarnitine/free carnitine ratio. The plasma short-chain acylcarnitine concentration increased by a factor of two to three during exercise, and was not significantly different from the values at rest 40 min after completion of exercise. Thus, the current studies illustrate the close interaction between the CoA and carnitine pools in the exercising human skeletal muscle, and they underscore the important role of carnitine in maintaining the muscular CoASH content during exhaustive exercise.