Improving Cell Growth and Lipid Accumulation in Green Microalgae Chlorella sp via UV Irradiation
Improving Cell Growth and Lipid Accumulation in Green Microalgae Chlorella sp via UV Irradiation
复制标题
改善绿色微藻小球藻的细胞生长和脂质积累。
DOI：
10.1007/s12010-015-1521-6
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发表时间：
2015-04-01
影响因子：
3
通讯作者：
Ma, Fang
中科院分区：
文献类型：
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作者：
Liu, Shuyu;Zhao, Yueping;Ma, Fang
微藻生物量大，油脂含量高，是生产生物柴油的理想原料。为了获得这样的微藻，对小球藻进行紫外线照射诱导突变。分析了对照和紫外诱变菌株的生长特性、总氮和生化组成。与对照菌株相比，紫外诱变菌株的生物量提高了7.6%，并表现出更高的生长速率。UV突变株的脂质含量表现出不同程度的增加，并在第15天达到最大值28. 1%。此外，UV突变菌株的脂质生产率显示出期望的增加。氮素消耗和乙酰辅酶A羧化酶（ACC）活性对紫外线诱导的油脂合成有促进作用。这些结果表明，紫外诱变是提高小球藻作为生物柴油潜在原料的可能性的有效方法。
Microalgae with high biomass and high lipid content are the ideal feedstock for biodiesel production. To obtain such microalgae, ultraviolet (UV) irradiation was applied to Chlorella sp. to induce mutagenesis. The growth characteristics, total nitrogen (TN), and biochemical compositions of the control and UV mutation strains were analyzed. Compared to the control strain, the biomass for the UV mutation strain was 7.6 % higher and it presented a higher growth rate. The lipid content of the UV mutation strain showed different levels of increase and reached the maximum value of 28.1 % on day 15. Furthermore, the lipid productivity of the UV mutation strain showed a desired increase. The nitrogen consumption and Acetyl-CoA carboxylase (ACC) activity contributed to the lipid production by UV. All these results indicate that UV mutagenesis is an efficient method to improve probability for using Chlorella sp. as the potential raw material for biodiesel production.