Ultraviolet B-induced apoptosis of human skin fibroblasts involves activation of caspase-8 and-3 with increased expression of vimentin
Ultraviolet B-induced apoptosis of human skin fibroblasts involves activation of caspase-8 and-3 with increased expression of vimentin
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DOI：
10.1111/j.1600-0781.2010.00522.x
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发表时间：
2010-08-01
影响因子：
2.6
通讯作者：
Wang, Baoxi
中科院分区：
文献类型：
--
作者：
Xu, Haoxiang;Yan, Yan;Wang, Baoxi
背景高剂量的紫外线B（UVB）照射后，细胞发生凋亡。Caspase-8和Caspase-3是许多细胞凋亡的关键介质。波形蛋白是一种重要的细胞骨架成分，可被caspase-3、-6、-7和-8切割。细胞凋亡是通过半胱天冬酶触发的波形蛋白的蛋白水解促进的。在这项研究中，我们探讨了Caspase-8和-3和波形蛋白表达的变化在UVB诱导的人皮肤成纤维细胞凋亡的作用。方法皮肤成纤维细胞照射150 mJ/cm 2 UVB和细胞死亡监测的3-（4，5）-二甲基噻唑（-Z-γ 1）-3，5-二苯基四氮唑溴化物测定和Hoechst染色。Caspase-8和caspase-3活性测定。免疫荧光和Western blot检测波形蛋白表达。结果150 mJ/cm 2 UVB照射可激活Caspase-8和Caspase-3。UVB照射诱导成纤维细胞凋亡后，Caspase-8和Caspase-3活性呈时间依赖性变化，且在此过程中Caspase-8和Caspase-3相互作用。然而，他们的底物，波形蛋白，表现出增强的表达，随着时间的推移后UVB照射。ConclusionsUVB触发的成纤维细胞凋亡依赖于caspase-8和-3的活化与波形蛋白的表达增加。
BackgroundAfter irradiation with a high dose of ultraviolet B (UVB), cells undergo apoptosis. Caspase-8 and -3 are key mediators of apoptosis in many cells. Vimentin, an important cytoskeleton component, can be cleaved by caspase-3, -6, -7 and -8. Cell apoptosis is promoted via caspase-triggered proteolysis of vimentin. In this study, we explored the roles of caspase-8 and -3 and the changes in vimentin expression in UVB-induced apoptosis of human dermal fibroblasts.MethodsSkin fibroblasts were irradiated with 150 mJ/cm2 UVB and cell death was monitored by the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide assay and Hoechst staining. Caspase-8 and -3 activities were detected by the caspase activity assay. Vimentin expression was assessed by immunofluorescence and Western blot.ResultsCaspase-8 and -3 were activated by 150 mJ/cm2 UVB irradiation. Caspase-8 and -3 activities changed in a time-dependent way after UVB irradiation to induce apoptosis of fibroblasts, and caspase-8 and -3 interacted with each other in this process. However, their substrate, vimentin, showed an enhanced expression over time after UVB irradiation.ConclusionsUVB-triggered apoptosis of fibroblasts was dependent on the activation of caspase-8 and -3 with an increased expression of vimentin.