Lycii cortex radicis extract inhibits glioma tumor growth in vitro and in vivo through downregulation of the Akt/ERK pathway
Lycii cortex radicis extract inhibits glioma tumor growth in vitro and in vivo through downregulation of the Akt/ERK pathway
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DOI：
10.3892/or.2012.1637
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发表时间：
2012-05-01
期刊：
影响因子：
4.2
通讯作者：
Kim, Ki Hyung
中科院分区：
文献类型：
--
作者：
Jeong, Ji Cheon;Kim, Saeng Jae;Kim, Ki Hyung
本研究旨在探讨朝鲜族传统草药-朝鲜族传统草药--朝鲜族传统草药-朝鲜族传统草药--朝鲜族传统草药-朝鲜族传统草药LCR提取物以剂量和时间依赖性方式导致凋亡性细胞死亡。LCR提取物诱导的细胞死亡与活性氧（ROS）的产生有关。Western blot分析显示LCR提取物可下调Akt和ERK的表达。LCR诱导的细胞死亡被阻止与Akt和MEK的组成型活性形式的转染。在皮下U87 MG异种移植模型中口服施用LCR提取物减少了胶质瘤肿瘤体积。总之，这些发现表明LCR提取物通过涉及ROS产生、Akt和ERK下调以及体外半胱天冬酶活化的机制导致人神经胶质瘤细胞死亡，并减少体内神经胶质瘤肿瘤生长。这些数据表明，LCR提取物可能作为一个潜在的治疗剂的恶性胶质母细胞瘤。
Lycii cortex radicis (LCR) is a traditional Korean medicinal herb. The present study was undertaken to examine the effect of an LCR extract on glioma cell growth and to determine its molecular mechanism in U87MG human glioma cells. The LCR extract resulted in apoptotic cell death in a dose- and time-dependent manner. The LCR extract-induced cell death was associated with generation of reactive oxygen species (ROS). Western blot analysis showed that the LCR extract caused downregulation of A kt and ERK. The LCR-induced cell death was prevented by transfection with the constitutively active forms of Akt and MEK. Oral administration of LCR extracts in subcutaneous U87MG xenograft models reduced glioma tumor volume. Taken together, these findings suggest that the LCR extract results in human glioma cell death through mechanisms involving ROS generation, downregulation of Akt and ERK, and caspase activation in vitro and reduces glioma tumor growth in vivo. These data suggest that the LCR extract may serve as a potential therapeutic agent for malignant human glioblastomas.